Contribution of Cyanide-Insensitive Respiratory Pathway, Catalyzed by the Alternative Oxidase, to Citric Acid Production in Aspergillus niger
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概要
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In Aspergillus niger, a cyanide (CN)-and antimycin A-insensitive and salicylhydroxamic acid (SHAM)-sensitive respiratory pathway exists besides the cytochrome pathway and is catalyzed by the alternative oxidase (AOX). In this study, A. niger WU-2223L, a citric acidproducing strain, was cultivated in a medium containing 120 g/l of glucose, which is the concentration usually needed for citric acid production, and the effects of 2% (v/v) methanol, an inducer of citric acid, 2μM antimycin A, and 1 mM SHAM on AOX activities and citric acid production were investigated. The AOX activity, measured as duroquinol oxidase, was localized in the purified mitochondria regardless of the presence of any additives. When WU-2223L was cultivated with antimycin A or methanol, both citric acid production and citric acid productivity, shown as the ratio of production per mycelial dry weight, increased with the increase of both the activity of AOX and the rate of CN-insensitive and SHAM-sensitive respiration. On the other hand, when WU-2223L was cultivated with SHAM, an inhibitor of AOX, the CN-insensitive and SHAM-sensitive respiration was not detected and the citric acid production and the productivity drastically decreased, although mycelial growth was not affected. These results clearly indicated that the CN-insensitive and SHAM-sensitive respiration catalyzed by AOX, localized in the mitochondria, contributed to citric acid production by A. niger.
- 社団法人日本農芸化学会の論文
- 2000-10-23
著者
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Kino Kuniki
Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Mizuno Makoto
Department of Bioproduction,Faculty of Bioindustry, Tokyo University of Agriculture
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Kino K
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Kino Kuniki
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Kino Kuniki
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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YODA MASASHI
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Usami S
Waseda Univ. Tokyo Jpn
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Usami S
Waseda Univ.
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Usami Shoji
Dept. Biol. Fac. Sci. Nagoya Univ.
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SHIMIZU Hideki
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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SUGANO Shuji
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Sugano Shuji
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Kino Kuniki
Dep. Of Applied Chemistry Fac. Of Sci. And Engineering Waseda Univ. 3-4-1 Ohkubo Shinjuku-ku Tokyo 1
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Mizuno Makoto
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Yoda Masashi
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Shimizu Hideki
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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