A Novel L-Amino Acid Ligase from Bacillus subtilis NBRC3134 Catalyzed Oligopeptide Synthesis

スポンサーリンク

概要

• 論文の詳細を見る

• L-Amino acid ligase catalyzes dipeptide synthesis from unprotected L-amino acids in an ATP-dependent manner. We have purified a new L-amino acid ligase, RizA, which synthesizes dipeptides whose N-terminus is Arg, from Bacillus subtilis NBRC3134, a microorganism that produces a rhizocticin peptide antibiotic. It was suggested that RizA is probably involved in rhizocticin biosynthesis. In this study, we performed sequence analysis of unknown regions around rizA, and newly identified a gene that encodes a protein that possesses an ATP-grasp motif upstream of rizA. This gene was designated rizB, and its recombinant protein was prepared. Recombinant RizB synthesized homo-oligomers of branched-chain L-amino acids and L-methionine consisting of two to five amino acids in an ATP-dependent manner. RizB also synthesized various heteropeptides. Further examination showed that RizB might elongate a peptide chain at the N-terminus. This is the first report on an L-amino acid ligase catalyzing oligopeptide synthesis.

• 社団法人 日本農芸化学会の論文
• 2010-01-23

著者

• Kino Kuniki

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Arai Toshinobu

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• TATEIWA Daisuke

  Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University

• Tateiwa Daisuke

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

関連論文

• Identification and characterization of a novel L-amino acid ligase from Photorhabdus luminescens subsp. laumondii TT01(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Dibenzothiophene Desulfurizing Enzymes from Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B : Purification, Characterization and Overexpression(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Gene Cloning and Characterization of Mycobacterium phlei Flavin Reductase Involved in Dibenzothiophene Desulfurization(Enzymology, Protein Engineering, and Enzyme Technology)
• Cloning and Expression of Aspergillus niger icdA Gene Encoding Mitochondrial NADP^+-Specific Isocitrate Dehydrogenase
• Biodesulfurization of Dibenzothiophene and Its Derivatives through the Selective Cleavage of Carbon-Sulfur Bonds by a Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B
• Enzymatic Synthesis of l-Menthyl α-Maltoside and l-Menthyl α-Maltooligosides from l-Menthyl α-Glucoside by Cyclodextrin Glucanotransferase
• Continuous Degradation of Dimethyl Sulfoxide to Sulfate Ion by Hyphomicrobium denitrificans WU-K217(ENVIRONMENTAL BIOTECHNOLOGY)
• Enzymatic Synthesis of α-Arbutin by α-Anomer-Selective Glucosylation of Hydroquinone Using Lyophilized Cells of Xanthomonas campestris WU-9701
• α-Anomer-Selective Glucosylation of (+)-Catechin by the Crude Enzyme, Showing Glucosyl Transfer Activity, of Xanthomonas campestris WU-9701
• α-Anomer-Selective Glucosylation of Menthol with High Yield through a Crystal Accumulation Reaction Using Lyophilized Cells of Xanthomonas campestris WU-9701
• Contribution of Cyanide-Insensitive Respiratory Pathway, Catalyzed by the Alternative Oxidase, to Citric Acid Production in Aspergillus niger
• Selective α-Glucosylation of Eugenol by α-Glucosyl Transfer Enzyme of Xanthomonas campestris WU-9701(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• A Novel L-Amino Acid Ligase from Bacillus licheniformis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Thermostable ATP Regeneration System Using Polyphosphate Kinase from Thermosynechococcus elongatus BP-1 for D-Amino Acid Dipeptide Synthesis(BIOCHEMICAL ENGINEERING)
• Substrate Specificity of Thermostable D-Alanine-D-alanine ligase from Thermotoga maritima ATCC 43589
• D-Amino Acid Dipeptide Production Utilizing D-Alanine-D-Alanine Ligases with Novel Substrate Specificity(Enzymology, Protein Engineering, and Enzyme Technology)
• Expression of Alternative Oxidase Gene (aox1) at the Stage of Single-Cell Conidium in Citric Acid-Producing Aspergillus niger(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Expression Analysis of Alternative Oxidase Gene (aox1) with Enhanced Green Fluorescent Protein as Marker in Citric Acid-Producing Aspergillus niger(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Isolation of Dimethyl Sulfone-Degrading Microorganisms and Application to Odorless Degradation of Dimethyl Sulfoxide
• Oxidative Degradation of Dimethyl Sulfoxide by Cryptococcus humicolus WU-2, a Newly Isolated Yeast(ENVIRONMENTAL BIOTECHNOLOGY)
• Enhancement of L-tryptophan 5-hydroxylation activity by structure-based modification of L-phenylalanine 4-hydroxylase from Chromobacterium violaceum
• Enhancement of L-tryptophan 5-hydroxylation activity by structure-based modification of L-phenylalanine 4-hydroxylase from Chromobacterium violaceum(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• A New Method of Synthesis of Alkyl β-Glycosides Using Sucrose as Sugar Donor
• Enzymatic Synthesis of α-Anomer-Selective D-Glucosides Using Maltose Phosphorylase
• Novel Reactivity of Dibenzothiophene Monooxygenase from Bacillus subtilis WU-S2B
• Biocatalytic Synthesis of Dihydroxynaphthoic Acids by Cytochrome P450 CYP199A2
• Structure-Based Modification of D-Alanine-D-Alanine Ligase from Thermotoga maritima ATCC 43589 for Depsipeptide Synthesis
• A Novel L-Amino Acid Ligase from Bacillus subtilis NBRC3134, a Microorganism Producing Peptide-Antibiotic Rhizocticin
• A Novel L-Amino Acid Ligase Is Encoded by a Gene in the Phaseolotoxin Biosynthetic Gene Cluster from Pseudomonas syringae pv. phaseolicola 1448A
• New L-Amino Acid Ligases Catalyzing Oligopeptide Synthesis from Various Microorganisms
• A Novel L-Amino Acid Ligase from Bacillus subtilis NBRC3134 Catalyzed Oligopeptide Synthesis
• Application of protein N-terminal amidase in enzymatic synthesis of dipeptides containing acidic amino acids specifically at the N-terminus(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Biological Reduction of Ferric Iron by Iron- and Sulfur-oxidizing Bacteria
• Application of protein N-terminal amidase in enzymatic synthesis of dipeptides containing acidic amino acids specifically at the N-terminus

もっと見る 閉じる

スポンサーリンク