Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

• 論文の詳細を見る

• Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55℃ and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.

• 社団法人日本生物工学会の論文

著者

• Kino Kuniki

  Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University

• Izumi Yoshikazu

  Dep. Of Biotechnology Tottori Univ.

• KIRIMURA Kohtaro

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• Kino K

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Kino Kuniki

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Kino Kuniki

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Takahashi Shusuke

  Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University

• Furuya Toshiki

  Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University

• Ishii Yoshitaka

  Institute for Biomedical Engineering, Consolidated Research Institute for Advanced Science and Medic

• Ishii Yoshitaka

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• Kino Kuniki

  Dep. Of Applied Chemistry Fac. Of Sci. And Engineering Waseda Univ. 3-4-1 Ohkubo Shinjuku-ku Tokyo 1

• Ishii Yoshitaka

  Institute For Biomedical Engineering Consolidated Research Institute For Advanced Science And Medica

• Kirimura Kohtaro

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Furuya Toshiki

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Takahashi Shusuke

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Ishii Yoshitaka

  Bio-refining Process Laboratory Advanced Technology And Research Institute Petroleum Energy Center

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