Intraspecific Protoplast Fusion of Citric Acid-Producing Strains of Aspergillus niger
スポンサーリンク
概要
- 論文の詳細を見る
Intraspecific protoplast fusion was carried out between different citric acid-producing strains of Aspergillus niger. Protoplasts prepared from the mycelia of auxotrophic mutants were treated in 30%(w/v)polyethylene glycol solution containng 0.01 M CaCl_2 and 0.5 M KCl, and fusion frequencies were 2.0-6.3%. Fusants were heterokaryons and in some cases formed sectors of the prototroph. Heterodiploids were induced from a heterokaryon by d-camphor treatment. Citric acid productivity of one heterodiploid was intermediate between those of parent strains in both shaking and solid cultures.
- 社団法人日本生物工学会の論文
- 1986-12-25
著者
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Usami S
Waseda Univ. Tokyo Jpn
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Usami Shoji
Dept. Biol. Fac. Sci. Nagoya Univ.
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YAGUCHI TAKASHI
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Yaguchi Takashi
Drug Discovery Pharmaceutical Research Center
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Yaguchi Takashi
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Yaguchi Takashi
Drug Discovery Pharmaceutical Research Center Meiji Seika Kaisha Ltd.
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Kirimura Kohtaro
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Kirimura K
Waseda Univ. Tokyo Jpn
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