Improvement of Docosahexaenoic Acid Production in a Culture of Thraustochytrium aureum by Medium Optimization
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概要
- 論文の詳細を見る
Growth optimization of a docosahexaenoic acid (DHA) producing marine fungus, Thraustochytrium aureum (ATCC 34304), resulted in cell productivity of 5.7 g/l and 460 mg/l of total lipid in 69 h flask culture, almost twice the level of cell productivity obtained for the strain reported previously. The lipid extracted from the cells was primarily composed of triacylglycerol and contained about 40% DHA. The fermentor culture showed a lower growth rate than the flask culture indicating growth inhibition due to mechanical stirring. Cells grown in the fermentor showed a coagulant tendency. ATCC 34304,in culture, seems to reproduce asexually and the life cycle of the cells consists of a zoospore stage, trophic cell stage and sporangium stage. Zoospore release was observed once during the early growth phase throughout growth period both in flask and fermentor culture.
- 社団法人日本生物工学会の論文
- 1996-01-25
著者
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Yagi H
National Institute Of Bio-science And Human Technology
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KAMISAKA YASUSHI
National Institute of Bioscience and Human Technology
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NAKAHARA TORO
National Institute of Bioscience and Human Technology
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YOKOCHI TOSHIHIRO
National Institute of Bioscience and Human Technology
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Yaguchi Takashi
Drug Discovery Pharmaceutical Research Center
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Yaguchi Takashi
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Yaguchi Takashi
Drug Discovery Pharmaceutical Research Center Meiji Seika Kaisha Ltd.
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Yamaoka Masakazu
National Institute Of Bio-science And Human Technology
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Yamaoka Masakazu
National Institute Of Advanced Industrial Science And Technology
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IIDA IWAO
Department of Biological Science and Technology, Science University of Tokyo
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YAGI HISAAKI
National Institute of Bio-science and Human Technology
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SUZUKI OSAMU
(Present address)Faculty of Engineering, Hiroshima University
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Iida Iwao
Department Of Biological Science And Technology Science University Of Tokyo
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Nakajima Toshiaki
Biological Resources Laboratory National Institute Of Advanced Industrial Science And Technology (ai
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Nakahara Toro
National Institute Of Advanced Industrial Science & Technology
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Yamamura K
Osaka Univ. Osaka Jpn
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Yokochi T
National Inst. Advanced Industrial Sci. And Technol. Ibaraki
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Yokochi Toshihiro
National Institute Of Advanced Industrial Science & Technology
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Kamisaka Y
National Inst. Advanced Industrial Sci. And Technol. Ibaraki
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Kamisaka Yasushi
National Institute Of Advanced Industrial Science And Technology (aist)
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YAMAOKA Masakazu
National Chemical Laboratory for Industry
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