Purification of Extracellular Lipases from Trichosporon fermentans WU-C12
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概要
- 論文の詳細を見る
Two types of extracellular lipases (I and II) from Trichosporon fermentans WU-C12 were purified by acetone precipitation and successive chromatographies on Butyl-Toyopearl 650 M, Toyopearl HW-55F and Q-Sepharose FF. The molecular weight of lipase I was 53 kDa by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and 160 kDa by gel filtration, while that of lipase II was 55 kDa by SDS-PAGE and 60 kDa by gel filtration. For the hydrolysis of olive oil, the optimum pH and temperature of both the lipases were 5.5 and 35℃, respectively. The lipases showed stable activities after incubation at 30℃ for 24 h in a pH range from 4.0 to 8.0. The thermostability of lipase I for 30 min at a reaction pH of 5.5 was up to 40℃, while that of lipase II under the same conditions was up to 50℃. Both lipases could hydrolyze the 1-, 2-, and 3-positions of triolein, and cleave all three ester bonds, regardless of the position in the triglyceride.
- 社団法人日本生物工学会の論文
- 1994-05-25
著者
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Kino K
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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SHIMURA Susumu
Central Laboratory Lotte Co., Ltd.
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Usami S
Waseda Univ. Tokyo Jpn
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Usami Shoji
Dept. Biol. Fac. Sci. Nagoya Univ.
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CHEN JIACONG
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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Chen Jiacong
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Shimura S
Central Laboratory Lotte Co. Ltd.
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Shimura Susumu
Central Laboratory Lotte Co. Ltd.
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Shiota Sumiko
Laboratory Of Pathogenic Microbiology School Of Pharmacy Shujitsu University
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