Structure-Based Modification of D-Alanine-D-Alanine Ligase from Thermotoga maritima ATCC 43589 for Depsipeptide Synthesis
スポンサーリンク
概要
- 論文の詳細を見る
Depsipeptides are peptide-like polymers consisting of amino acids and hydroxy acids, and are expected to be new functional materials for drug-delivery systems and polymer science. In our previous study, D-alanyl-D-lactate, a type of depsipeptide, was enzymatically synthesized using D-alanine-D-alanine ligase from Thermotoga maritima ATCC 43589 (TmDdl) by Y207F substitution. Thereafter, in this study, further mutagenesis was introduced, based on structural comparison between TmDdl and a well-characterized D-alanine-D-alanine ligase from Escherichia coli. The S137A/Y207F mutant showed higher D-alanyl-D-lactate and lower D-alanyl-D-alanine synthesizing activity than the Y207F mutant. This suggests that substitution at the S137 residue contributes to product selectivity. Saturated mutagenesis on S137 revealed that the S137G/Y207F mutant showed the highest D-alanyl-D-lactate synthesizing activity. Moreover, the mutant showed broad substrate specificity toward D-amino acid and recognized D-lactate and D,L-isoserine as substrates. On the basis of these characteristics, various depsipeptides can be produced using S137G/Y207F-replaced TmDdl.
- 2011-04-23
著者
-
Kino K
Department Of Applied Chemistry School Of Science And Engineering Waseda University
-
Kino Kuniki
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
-
Kino Kuniki
Department Of Applied Chemistry School Of Science And Engineering Waseda University
-
Kino Kuniki
Dep. Of Applied Chemistry Fac. Of Sci. And Engineering Waseda Univ. 3-4-1 Ohkubo Shinjuku-ku Tokyo 1
-
Satake Ryoko
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
-
Sato Masaru
Department Of Applied Chemistry School Of Advanced Science And Engineering Faculty Of Science And En
-
NAKAGAWA Tomoki
Department of Applied Chemistry, School of Advanced Science and Engineering, Faculty of Science and
-
Nakagawa Tomoki
Department Of Applied Chemistry School Of Advanced Science And Engineering Faculty Of Science And En
関連論文
- Identification and characterization of a novel L-amino acid ligase from Photorhabdus luminescens subsp. laumondii TT01(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Dibenzothiophene Desulfurizing Enzymes from Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B : Purification, Characterization and Overexpression(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Gene Cloning and Characterization of Mycobacterium phlei Flavin Reductase Involved in Dibenzothiophene Desulfurization(Enzymology, Protein Engineering, and Enzyme Technology)
- Cloning and Expression of Aspergillus niger icdA Gene Encoding Mitochondrial NADP^+-Specific Isocitrate Dehydrogenase
- Biodesulfurization of Dibenzothiophene and Its Derivatives through the Selective Cleavage of Carbon-Sulfur Bonds by a Moderately Thermophilic Bacterium Bacillus subtilis WU-S2B
- Expansion Working of Brass Tubes by Plug Drawing
- The Mechanism of Correcting the Eccentricity of Brass Tubes by Sinking
- Enzymatic Synthesis of l-Menthyl α-Maltoside and l-Menthyl α-Maltooligosides from l-Menthyl α-Glucoside by Cyclodextrin Glucanotransferase
- Continuous Degradation of Dimethyl Sulfoxide to Sulfate Ion by Hyphomicrobium denitrificans WU-K217(ENVIRONMENTAL BIOTECHNOLOGY)
- Enzymatic Synthesis of α-Arbutin by α-Anomer-Selective Glucosylation of Hydroquinone Using Lyophilized Cells of Xanthomonas campestris WU-9701
- α-Anomer-Selective Glucosylation of (+)-Catechin by the Crude Enzyme, Showing Glucosyl Transfer Activity, of Xanthomonas campestris WU-9701
- α-Anomer-Selective Glucosylation of Menthol with High Yield through a Crystal Accumulation Reaction Using Lyophilized Cells of Xanthomonas campestris WU-9701
- Determination of Nucleotide Sequence Related to the Plasmid Replication Region in Enterococcus faecalis and Its Application to a New Shuttle Vector
- Purification and Characterization of a Novel β-Agarase from an Alkalophilic Bacterium, Alteromonas sp.E-1
- Anomer-Selective Glucosylation of l-Menthol Using Lyophilized Cells of Saccharomyces cerevisiae
- Anomer Selective Formation of l-Menthyl α-D-Glucopyranoside by α-Glucosidase-catalyzed Reaction
- Purification of Extracellular Lipases from Trichosporon fermentans WU-C12
- Lipase Production from Hydrocarbons by Trichosporon fermentans WU-C12 in the Presence of Surfactants
- Lipase Production by Trichosporon fermentans WU-C12, a Newly Isolated Yeasts
- Production of β-Fructofuranosidase Showing Fructose-Transferring Activity by Penicillium frequentans (P. glabrum)
- Ester Synthesis by a Crude Lipase of Rhizopus oligosporus in an Aqueous System
- Contribution of Cyanide-Insensitive Respiratory Pathway, Catalyzed by the Alternative Oxidase, to Citric Acid Production in Aspergillus niger
- Cloning and Sequencing of the Chromosomal DNA and cDNA Encoding the Mitochondrial Citrate Synthase of Aspergillus niger WU-2223L
- Selective α-Glucosylation of Eugenol by α-Glucosyl Transfer Enzyme of Xanthomonas campestris WU-9701(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- P-493 Studies on Constituents of Erythrina variegata (Leguminosae)
- A new amide from the leaves and twigs of Litsea auriculata
- A Novel L-Amino Acid Ligase from Bacillus licheniformis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Phytochemical Flavones with the Antibacterial Activity against Methicillin-Resistant Staphylococcus aureus
- Enzymatic Synthesis of α-Anomer-Selective D-Glucosides Using Maltose Phosphorylase
- Thermostable ATP Regeneration System Using Polyphosphate Kinase from Thermosynechococcus elongatus BP-1 for D-Amino Acid Dipeptide Synthesis(BIOCHEMICAL ENGINEERING)
- Substrate Specificity of Thermostable D-Alanine-D-alanine ligase from Thermotoga maritima ATCC 43589
- D-Amino Acid Dipeptide Production Utilizing D-Alanine-D-Alanine Ligases with Novel Substrate Specificity(Enzymology, Protein Engineering, and Enzyme Technology)
- Expression of Alternative Oxidase Gene (aox1) at the Stage of Single-Cell Conidium in Citric Acid-Producing Aspergillus niger(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Expression Analysis of Alternative Oxidase Gene (aox1) with Enhanced Green Fluorescent Protein as Marker in Citric Acid-Producing Aspergillus niger(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Evaluation of [111]-Textured Cu Layer Formed on Thin Nb Barrier Layer on SiO_2(Session 8A Silicon Devices V,AWAD2006)
- Evaluation of [111]-Textured Cu Layer Formed on Thin Nb Barrier Layer on SiO_2(Session 8A Silicon Devices V)
- Isolation of Dimethyl Sulfone-Degrading Microorganisms and Application to Odorless Degradation of Dimethyl Sulfoxide
- Oxidative Degradation of Dimethyl Sulfoxide by Cryptococcus humicolus WU-2, a Newly Isolated Yeast(ENVIRONMENTAL BIOTECHNOLOGY)
- Enhancement of L-tryptophan 5-hydroxylation activity by structure-based modification of L-phenylalanine 4-hydroxylase from Chromobacterium violaceum
- Enhancement of L-tryptophan 5-hydroxylation activity by structure-based modification of L-phenylalanine 4-hydroxylase from Chromobacterium violaceum(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- A New Method of Synthesis of Alkyl β-Glycosides Using Sucrose as Sugar Donor
- A New Method of Synthesis of Alkyl β-Glycosides Using Sucrose as Sugar Donor
- Enzymatic Synthesis of α-Anomer-Selective D-Glucosides Using Maltose Phosphorylase
- Simultaneous determination of catechins in human saliva by high-performance liquid chromatography
- Novel Reactivity of Dibenzothiophene Monooxygenase from Bacillus subtilis WU-S2B
- Biocatalytic Synthesis of Dihydroxynaphthoic Acids by Cytochrome P450 CYP199A2
- Structure-Based Modification of D-Alanine-D-Alanine Ligase from Thermotoga maritima ATCC 43589 for Depsipeptide Synthesis
- A Novel L-Amino Acid Ligase from Bacillus subtilis NBRC3134, a Microorganism Producing Peptide-Antibiotic Rhizocticin
- Cross-Sectional Observation of Fe_3O_Fe_2O_3 Intermediate Thin Films
- A Novel L-Amino Acid Ligase Is Encoded by a Gene in the Phaseolotoxin Biosynthetic Gene Cluster from Pseudomonas syringae pv. phaseolicola 1448A
- New L-Amino Acid Ligases Catalyzing Oligopeptide Synthesis from Various Microorganisms
- A Novel L-Amino Acid Ligase from Bacillus subtilis NBRC3134 Catalyzed Oligopeptide Synthesis
- Study on Tube-Expanding by Drawing of Phosphor Deoxidized Copper Tubes : Fourth Report : Relations between Contact Length and Dimensional Accuracy
- Study on Tube-Expanding by Drawing of Phosphor-Deoxidized Copper Tubes : Third Report : Relations between Tool Shapes and Changes in Wall-thickness
- Barrier Properties of Thin ZrNx Films Prepared by Radical-Assisted Surface Reaction
- Atomic Layer Deposition of Thin VNx Film from Tetrakis(diethylamido)vanadium Precursor
- Study on Precision Clad Tube Working by Tube-Expanding
- Application of Extremely Thin ZrN Film as Diffusion Barrier between Cu and SiOC
- Reactively Sputtered Nanocrystalline ZrN Film as Extremely Thin Diffusion Barrier between Cu and SiO2
- Preferentially Oriented Cu[111] Layer Formed on Thin Nb Barrier on SiO2
- Application of protein N-terminal amidase in enzymatic synthesis of dipeptides containing acidic amino acids specifically at the N-terminus(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- EFFECT OF CONSECUTIVE DOSING OF THIAMPHENICOL ON ITS OWN SERUM CONCENTRATION AND LIVER DRUG METABOLIZING ENZYME ACTIVITIES
- Reactions of Haloferrocenes. IV. The Condensation of Haloferrocenes with Various Carboxylic Acids in the Presence of Copper(I) Oxide
- Biological Reduction of Ferric Iron by Iron- and Sulfur-oxidizing Bacteria
- Biochemical and pharmacological analysis of 2-((2-dimethylaminobenzyl)sulfinyl)benzimidazole (NC-1300), a new proton pump inhibitor.
- Ring-enlargement of methoxynaphthalenes with dichlorocarbene to benzotropone derivatives.
- The Reaction of Bromobenzene with Copper(I) Chloride - Pyridine Complex in Nitrobenzene
- Reactions of Haloferrocenes. III. The Reaction of Haloferrocenes with Copper(I) Benzenethiolate, Thiocyanate, Acetate, and Benzoate
- Syntheses and NMR Spectra of the Substituted Methylferrocenes
- Reactions of Haloferrocenes. II. Kinetic Studies of the Reaction of Haloferrocene with Copper (I) Chloride - Pyridine Complex
- Bromination of methoxybiphenylenes.
- Reaction of 1,2-dimethoxybiphenylene with dichlorocarbene. Chemical detection of the bond fixation in biphenylene derivatives.
- The syntheses of 4- and 5-(.ALPHA.- and .BETA.-naphthyl)tropolones.
- Application of protein N-terminal amidase in enzymatic synthesis of dipeptides containing acidic amino acids specifically at the N-terminus