Cellular Localization of the Constitutive β-Glucosidase in Trichoderma viride

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概要

• 論文の詳細を見る

• The cellular localization of the constitutive β-glucosidase in the mycelia of Trichoderma viride was investigated by cell fractionation using disrupted mycelia and mycelial protoplasts. In 2-d-old mycelia, approximately 86% of the cellular enzyme activity was detected in the fraction containing cytosol, plasma membrane, and periplasm, and 14% in that containing cell wall. With incubation of the mycelia in a lytic enzyme solution, the β-glucosidase was released rapidly before protoplast formation was observed. After 4 h of incubation in the lytic enzyme solution, only 4% of β-glucosidase activity was detected in the protoplasts recovered. These results indicated that most of the constitutive β-glucosidase was on the cell surface, especially in the cell wall and periplasm.

• 社団法人日本生物工学会の論文
• 1990-09-25

著者

• Usami Shoji

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• KIRIMURA Kohtaro

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• Usami S

  Waseda Univ. Tokyo Jpn

• Usami S

  Waseda Univ.

• Usami Shoji

  Dept. Biol. Fac. Sci. Nagoya Univ.

• MORIKAWA SATOSHI

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• IMURA MASATOSHI

  (Present address)Kirin Brewery CO., Ltd.,

• Imura Masatoshi

  (present Address)kirin Brewery Co. Ltd.

• Kirimura Kohtaro

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Kirimura Kohtaro

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

• Usami Shoji

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Kirimura K

  Waseda Univ. Tokyo Jpn

• Morikawa Satoshi

  Department Of Applied Chemistry School Of Science And Engineering Waseda University

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