Improvement in Citric Acid Production by Haploidization of Aspergillus niger Diploid Strains
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概要
- 論文の詳細を見る
Segregation of diploid strains by a haploidizing agent was used to improve citric acid-producing strains of Aspergillus niger. Stable diploid strains were obtained via protoplast fusion between two citric acid-producing strains from different genealogies, one for shaking culture and the other for solid culture. Diploid strains were treated by benomyl as a haploidizing agent, and many segregants were obtained. Prototrophic segregants were selected and their haploidy was confirmed by their conidial size and DNA contents. The prototrophic segregants were very varible in their citric acid productivities, some of them betteer either in shaking culture or in solid culture than both the parental strains. The presence of methanol stimulated citric acid production by the parental and the diploid strains However, all prototrophic segregants derived from one diploid strain had higher productivities in solid cultures without methanol than in those with methanol.
- 社団法人日本生物工学会の論文
- 1988-08-25
著者
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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LEE Sung
Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center
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Lee S
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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KIRIMURA Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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NAKAJIMA IWAO
Department of Applied Chemistry, School of Science and Engineering, Waseda University
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KAWABE SEIICHIRO
(Present address)Department of Fundamental Natural Science, Okayama University of Science
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Lee Sung
Department Of Aerospace Engineering Korea Advanced Institute Of Science And Technology
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Usami S
Waseda Univ. Tokyo Jpn
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Usami S
Waseda Univ.
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Usami Shoji
Dept. Biol. Fac. Sci. Nagoya Univ.
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Kirimura Kohtaro
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Kirimura Kohtaro
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Nakajima Iwao
Department Of Applied Chemistry School Of Science And Engineering Waseda University
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Kirimura K
Waseda Univ. Tokyo Jpn
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Kawabe S
Okayama Univ. Science Okayama Jpn
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