Transgenic Rice Plants Expressing a Bacillus subtilis Protoportphyrinogen Oxidase Gene Are Resistant to Diphenyl Ether Herbicide Oxyfluorfen
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概要
- 論文の詳細を見る
Protoporphyrinogen oxidase(Protox), the penultimate step enzyme of the branch point for the biosynthetic pathway of Chl and hemes, is the target site of action of diphenyl ether(DPE)herbicides. However, Bacillus subtilis Protox is known to be resistant to the herbicides. In order to develop the herbicide-resistant plants, the transgenic rice plants were generated via expression of B.subtilis Protox gene under ubiquitin promoter targeted to the cytoplasm or to the plastid using Agrobacterium-mediated gene transformation. The integration and expression of the transgene were investigated at T_0 generation by DNA and RNA blots. Most transgenic rice plants revealed one copy transgene insertion into the rice genome, but some with 3 copies. The expression levels of B.subtilis Protox mRNA appeared to correlate with the copy number. Furthermore, the plastidal transgenic lines exhibited much higher expression of the Protox mRNA than the cytoplasmic transgenic lines. The transgenic plants expressing the B.subtilis Protox gene at T_0 generation were found to be resistant to oxyfluorfen when judged by cellular damage with respect to cellular leakage, Chl loss, and lipid peroxidation. The transgenic rice plants targeted to the plastid exhibited higher resistance to the herbicide than the transgenic plants targeted to the cytoplasm. In addition, possible resistance mechanisms in the transgenic plants to DPE herbicides are discussed.
- 日本植物生理学会の論文
著者
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LEE Sung
Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center
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Lee Hee
School of Plant Science, Seoul National University
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Back Kyoungwhan
Department Of Biotechnology Biotechnology Research Institute Chonnam National University
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Jung Sunyo
Scigen Harvest Research Center
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Han Sung
Department Of Agronomy Chonnam National University
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Lee Sung
Department Of Aerospace Engineering Korea Advanced Institute Of Science And Technology
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JEON Jong
Department of Material Science and Engineering, Pusan National University
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Lee Hee
Biotechnology Research Institute, Chonnam National University
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Chung Jung
Department of Agronomy, Chonnam National University
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Han Oksoo
Department of Genetic Engineering, Chonnam National University
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Guh Ja
Department of Agronomy, Chonnam National University
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An Gynheung
Department of Life Science, Pohang University of Science and Technology
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Lee H
School Of Plant Science Seoul National University
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Han O
Chonnam National Univ. Gwangju Kor
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Han Oksoo
Department Of Genetic Engineering Biotechnology Research Institute Chonnam National University
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Guh J
Institute Of Agricultural Science And Technology Chonnam National University
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Jeon Jong
Department Of Life Science Pohang University Of Science And Technology
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An Gynheung
Department Of Life Science Pohang University Of Science And Technology
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Lee Sung
Department Of Agronomy Chonnam National University
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