Selective Cleavage of the Two C-S Bonds in Asymmetrically Alkylated Dibenzothiophenes by Rhodococcus erythropolis KA2-5-1

概要

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The Rhodococcus erythropolis strain KA2-5-1 was characterized by its ability to cleave carbon-sulfur bonds in the dibenzothiophene(DBT)ring by asymmetrically alkyl substitution, such as C_2-DBTs(e.g., dimethyl and ethyl DBTs)and C_3-DBTs(e.g., trimethyl and propyl DBTs), which are known to remain in hydrodesulfurization-treated diesel fuels. After treatment by solid-phase extraction(SPE)of solvents from microbial reactions of alkylated DBTs(Cx-DBTs), we used gas chromatography(GC), GC-atomic emission detection, GC-mass spectrometry and ^1H nuclear magnetic resonance spectroscopy to identify and quantitatively evaluate the Cx-DBT metabolites. Molar ratios of metabolic isomers of the desulfurization products suggested that resting-cell reactions of KA2-5-1 against these Cx-DBTs occurrs through specific carbon-sulfur-bond-targeted cleavages, yielding alkylated hydroxybiphenyls, and that the manner of the attack on the DBT skeleton is affected not only by the position but also by the number and length of the alkyl substituents.
公益社団法人日本生物工学会の論文
2001-07-25