Gene Encoding a Dextransucrase-like Protein in Leuconostoc mesenteroides NRRL B-512F

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概要

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• A gene, dsrT, encoding a dextransucrase-like protein was isolated from the genomic DNA libraries of Leuconostoc mesenteroides NRRL B-512F dextransucraselike gene. The gene was similar to the intact open reading frames of the dextransucrase gene dsrS of L. mesenteroides NRRL B-512F, dextransucrase genes of strain NRRL B-1299 and streptococcal glucosyltransferase genes, but was truncated after the catalytic domain, apparently by the deletion of five nucleotides. dsrT mRNA was produced in this strain L. mesenteroides when cells were grown in a sucrose medum, but at a level of 20% of that of dsrS mRNA. The molecular weight of the dsrT gene product was 150,000 by SDSPAGE. The product did not synthesize dextran, but had weak sucrose cleaving activity. The insertion of five nucleotides at the putative deletion point in dsrT resulted in an enzyme with a molecular weight of 210,000 and with dextransucrase activity.

• 社団法人日本農芸化学会の論文
• 2000-01-23

著者

• Kobayashi Miki

  Biochemicals Laboratory Mitsubishi Chemical Corporation

• Kobayashi Masatomo

  Inst. Phys. Chem. Res. (riken)

• Kobayashi M

  Graduate School Of Pharmaceutical Sciences Osaka University

• Kobayashi M

  Carbohydrate Science Laboratory National Food Research Institute Ministry Of Agriculture Forestry An

• MIZUNO Kouichi

  Institute of Applied Biochemistry, University of Tsukuba

• FUNANE Kazumi

  Carbohydrate Science Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry

• MIZUNO Kouichi

  Carbohydrate Science Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry

• TAKAHARA Hidenari

  Department of Resource Biology Faculty of Agriculture, Ibaraki University

• KOBAYASHI Mikihiko

  Akita Research Institute of Food and Brewing

• Takahara H

  Department Of Resource Biology Faculty Of Agriculture Ibaraki University

• Takahara Hidenari

  Department Of Applied Biological Resource Sciences School Of Agriculture Ibaraki University

• Funane K

  National Food Research Institute

• TAKAHARA Hidenari

  Department of Agricultural Chemistry, Faculty of Agriculture, Ibaraki University

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