Cloning, Expression, and Characterization of an Antifungal Chitinase from Leucaena leucocephala de Wit(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Chitinase cDNAs from Leucaena leucocephala seedlings were cloned by PCR amplification with degenerate primers based on conserved class I chitinase sequences and cDNA library screening. Two closely related chitinase cDNAs were sequenced and inferred to encode precursor proteins of 323 (KB1) and 326 (KB2) amino acids. Expression of the KB2 chitinase from a pET32a plasmid in Origami (DE3) Escherichia coli produced high chitinase activity in the cell lysate. The recombinant thioredoxin fusion protein was purified and cleaved to yield a 32-kDa chitinase. The recombinant chitinase hydrolyzed colloidal chitin with endochitinase-type activity. It also inhibited growth of 13 of the 14 fungal strains tested.
- 社団法人日本農芸化学会の論文
- 2003-04-23
著者
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FUJIMURA Tatsuhito
Institute of Agricultural and Forest Engineering, University of Tsukuba
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MIZUNO Kouichi
Institute of Applied Biochemistry, University of Tsukuba
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Sriyotha Poonsook
Institute Of Science Suranaree University Of Technology
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Mizuno Kouichi
Institute Of Agricultural And Forest Engineering University Of Tsukuba:(present Address)faculty Of B
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KAOMEK Mana
Institute of Science, Suranaree University of Technology
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CAIRNS James
Institute of Science, Suranaree University of Technology
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Kaomek Mana
Institute Of Science Suranaree University Of Technology:faculty Of Science And Technology Rajabhat I
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Cairns James
Institute Of Science Suranaree University Of Technology:biochemistry Laboratory Chulabhorn Research
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Mizuno Kouichi
Institute Of Agricultural And Forest Engineering University Of Tsukuba
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Fujimura Tatsuhito
Institute Of Agricultural And Forest Engineering University Of Tsukuba
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