Construction of a High-density AFLP and SSR Map Using Recombinant Inbred Lines of Cultivated×Weedy Soybean
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概要
- 論文の詳細を見る
A genetic linkage map of soybean was constructed by using a cross between a cultivar 'Keburi' and a weedy form of soybean 'Masshokutou Kou 502'. The parents differed in their ability in somatic embryogenesis. The mapping population consisted of 117 recombinant inbred lines (F_<11>), and generated 30 linkage groups, which covered 2089 cM, including 515 amplified-fragment-length polymorphism (AFLP) markers and 85 simple sequence repeat (SSR) markers. For AFLP analysis, a simple technique using small polyacrylamide gels combined with silver staining was applied, which enabled a single person to construct a map in a short time at a reasonable cost. Comparison of this map with previously published maps revealed that 1) the level of polymer-phism was the same as in a cultivar×cultivar cross; and 2) the cultivar×weed map was 13% shorter than the cultivar×wild map. The high-density map constructed in this study would contribute to the molecular mapping of quantitatively and qualitatively inherited characters from weedy forms of soybean.
- 日本育種学会の論文
著者
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FUJIMURA Tatsuhito
Institute of Agricultural and Forest Engineering, University of Tsukuba
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Takahata Yoshihito
Faculty Of Agnculture Lwate Untversity
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Choi Pilson
National Institute Of Agrobiological Sciences (nias):(present Address) Eugentech Korea Research Inst
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Ishikawa Atsuko
National Inst. Radiological Sci. Chiba Jpn
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Ishikawa Atsuko
National Institute Of Agrobiological Sciences:faculty Of Agriculture Iwate University
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Umezawa Taishi
Institute Of Agricultural And Forest Engineering University Of Tsukuba:(present Address) Riken Tsuku
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Komatsuda Takao
National Inst. Of Agrobiological Sciences (nias)
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Mano Yoshiro
National Institute of Agrobiological Sciences (NIAS)
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Odashima Masashi
Institute of Agricultural and Forest Engineering, University of Tsukuba
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Fujimura Tatsuhito
Institute Of Agricultural And Forest Engineering University Of Tsukuba
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Mano Y
National Institute Of Agrobiological Sciences (nias):(present Address) National Institute Of Livesto
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Odashima Masashi
Institute Of Agricultural And Forest Engineering University Of Tsukuba:(present Address) Iwate Agric
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Komatsuda Takao
National Inst. Agrobiological Sci.(nias) Ibaraki Jpn
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