Molecular Analysis of Maleate cis-trans Isomerase from Thermophilic Bacteria

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概要

• 論文の詳細を見る

• Several strains of thermophilic bacteria containing maleate cis-trans isomerase were isolated from soil samples and identified as Bacillus stearothermophilus, Bacillus circulans, Bacillus brevis, and Deleya halophila. The maleate cis-trans isomerase was purified and characterized from one of the isolated strains, B. stearothermophilus MI-102. The purified enzyme of strain MI-102 showed higher thermal stability than the enzyme of a mesophile, Alcaligenes faecalis IFO13111. The seven maleate cis-trans isomerase genes (maiA) of thermophile were cloned and sequenced. B. stearothemophilus MI-102 MaiA has 67% amino acid identity with A. faecalis MaiA. All eight amino acid sequences of maiA gene products had significant conserved regions containing cysteine residues, which were previously suggested to be involved in an active site of the enzyme. To probe the catalytic mechanism, three cysteine residues in the conserved regions of A. faecalis MaiA were replaced with serine by site-directed mutagenesis. The results suggest that Cys80 and Cys198 play important roles in the enzyme activity.

• 社団法人日本農芸化学会の論文
• 2000-03-23

著者

• Kobayashi Miki

  Biochemicals Laboratory Mitsubishi Chemical Corporation

• Goto Machio

  Hitachi Instrument Engineering Co. Ltd.

• Goto M

  Mie Univ. Tsu Jpn

• HATAKEYAMA Kazuhisa

  Biochemicals Laboratory, Tsukuba Research Center, Mitsubishi Chemical Corporation

• GOTO Makoto

  Biochemicals Laboratory, Tsukuba Research Center, Mitsubishi Chemical Corporation

• TERASAWA Masato

  Biochemicals Laboratory, Tsukuba Research Center, Mitsubishi Chemical Corporation

• YUKAWA Hideaki

  Biochemicals Laboratory, Tsukuba Research Center, Mitsubishi Chemical Corporation

• UCHIDA Yasukazu

  Biochemicals Laboratory, Mitsubishi Chemical Corporation

• Kobayashi Masatomo

  Inst. Phys. Chem. Res. (riken)

• Kobayashi M

  Graduate School Of Pharmaceutical Sciences Osaka University

• Kobayashi M

  Carbohydrate Science Laboratory National Food Research Institute Ministry Of Agriculture Forestry An

• Terasawa Masato

  Biochemicals Laboratory Mitsubishi Chemical Corporation

• Hatakeyama Kazuhisa

  Biochemicals Laboratory Mitsubishi Chemical Corporation

• Uchida Yasukazu

  Biochemicals Laboratory Mitsubishi Chemical Corporation

• Yukawa H

  Res. Inst. Innovative Technol. Earth Kyoto Jpn

• Yukawa Hideaki

  Biochemicals Laboratory, Mitsubishi Chemical Corporation

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