Replacement Recombination in Coryneform Bacteria: High Efficiency Integration Requirement for Non-methylated Plasmid DNA
スポンサーリンク
概要
- 論文の詳細を見る
We have developed a versatile system that permits efficient manipulation of coryneform bacteria by gene disruption and replacement. Circumventing the methyl-specific restriction system in certain of these bacteria by using non-methylated plasmid DNA was necessary. Integrative plasmid DNA containing an in vitro disrupted copy of the Brevibacterium flavum thr B gene was introduced into B. flavum at an efficiency of 2.4×10^2 integrants/μg. Integration occurred by homologous recombination either via a Campbell-like mechanism or via a double crossover event. Integrants were stable for over 32 generations.
- 社団法人日本農芸化学会の論文
- 1993-12-23
著者
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Kobayashi Miki
Biochemicals Laboratory Mitsubishi Chemical Corporation
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Kurusu Y
Ibaraki Univ. Ibaraki Jpn
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Kurusu Yasurou
Mitsubishi Petrochemical Co. Ltd. Tsukuba Research Center
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Vertes A.
Mitsubishi Petrochemical Co., Ltd., Tsukuba Research Center
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Hatakeyama Kazuhisa
Mitsubishi Petrochemical Co., Ltd., Tsukuba Research Center
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Inui Masayuki
Mitsubishi Petrochemical Co., Ltd., Tsukuba Research Center
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Kobayashi Miki
Mitsubishi Petrochemical Co., Ltd., Tsukuba Research Center
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Yukawa Hideaki
Mitsubishi Petrochemical Co., Ltd., Tsukuba Research Center
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Kobayashi Masatomo
Inst. Phys. Chem. Res. (riken)
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Kobayashi M
Graduate School Of Pharmaceutical Sciences Osaka University
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Kobayashi M
Carbohydrate Science Laboratory National Food Research Institute Ministry Of Agriculture Forestry An
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Hatakeyama Kazuhisa
Biochemicals Laboratory Mitsubishi Chemical Corporation
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Vertes A.
Mitsubishi Petrochemical Co. Ltd. Tsukuba Research Center
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Inui M
Res. Inst. Innovative Technol. Earth Kyoto Jpn
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Yukawa H
Res. Inst. Innovative Technol. Earth Kyoto Jpn
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