Mold Pectinase Modified with Dialdehyde Derivatives of Dextran and Cellulose
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概要
- 論文の詳細を見る
Chemical modification of mold pectinase with dextran- and cellulose-dialdehydes was examined to improve the enzyme characteristics. The modified pectinase with dextran-dialdehyde retained about 50% of the original activity, and more than 80% of the total amino groups were modified. HPLC gel filtration analysis showed an increase in molecular weight of the reaction product. Reaction with Cellulose-dialdehyde provided an immobilized form of pectinase. The immobilized pectinase was resistant to both acidic and alkaline pHs, and also acquired heat stability at 60℃. The optimum pH of the modified enzyme shifted from pH 4.5 to 5.0-5.5, and this enzyme had higher activity at neutral pH regions than the native enzyme. A rather low recovery of immobilized enzyme (14.5%) should be improved by the combination with various methods hitherto established.
- 社団法人日本農芸化学会の論文
- 1996-05-23
著者
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Kobayashi Miki
Biochemicals Laboratory Mitsubishi Chemical Corporation
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Kato Yasuhito
Agro & Fine Chemicals Research Laboratory Nippon Kayaku Co. Ltd.
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Kato Y
Azabu Univ. Sagamihara Jpn
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Kato Y
Tokaigakuen Women's College
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Kato Yoji
Faculty Of Education Hirosaki University
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Kobayashi Masatomo
Inst. Phys. Chem. Res. (riken)
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Kato Yoji
Laboratory Of Food Science Faculty Of Education Hirosaki University
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Kobayashi M
Graduate School Of Pharmaceutical Sciences Osaka University
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KOBAYASHI Mikihiko
National Food Research Institute
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CHIBA Yogo
National Food Research Institute
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FUNANE Kazumi
National Food Research Institute
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OHYA Setsuko
National Food Research Institute
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KATO YOSHIAKI
Osaka College of Pharmacy
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Kobayashi M
Carbohydrate Science Laboratory National Food Research Institute Ministry Of Agriculture Forestry An
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Funane K
National Food Research Institute
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