A Model System for Convenient Fluorescent Labeling of Sugar Chain in Taka-amylase A
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概要
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A convenient detection of sugar chains in Taka-amylase A (TAA) was done by using 40 μg of enzyme, where a decrease in the UV absorption of NaIO_4 during the periodate oxidation reaction was monitored. The periodate-oxidized sugar chain was labeled with a fluorescent reagent, N-1-ethylenediaminonaphthalene (EDAN), by incubation at pH 9.5 and 30℃ for 1h. The excess EDAN was removed by either quenching with o-phthaladehyde or Bio-Gel P-2 gel adsorption. Among the peptide fragments preparbd from the EDAN-labeled TAA, a fluorescent peptide corresponding to the sugar chain was distinguished by the ODS column. These results suggest that periodate oxidation and subsequent fluorescent labeling were useful for the sensitive analysis of various glycoprotein samples.
- 社団法人日本農芸化学会の論文
- 1997-11-23
著者
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SASAKI Yutaka
National Agricultural Research Center for Kyushu Okinawa Region
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KOBAYASHI Mikihiko
National Food Research Institute
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CHIBA Yogo
National Food Research Institute
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KOBAYSHI Mikihiko
National Food Research Institute
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