Characterization of Iron (II) : Comoplexes with β-Diketones and Monthio-β-diketone (Commemoration Issue Dedicated to Professor Tsunenobu Shigematsu on the Occasion of his Retirement)
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概要
- 論文の詳細を見る
Dextran-dialdehyde was attached to trypsin molecules by incubation at 30℃ and pH 8. 5. The cross-linking reaction was completed within a few hours, and the dextran-modified trypsin was isolated by gel filtration chromatography. The modified trypsin was resistant to auto-hydrolysis and a significant increases in thermal and pH stabilities was observed. Storage of modified trypsin at 4℃ under the optimum pH caused little loss of activity. The modified trypsin was resistant to organic solvents such as 10% ethanol and 0. 2% sodium dodecylsulfate. Moreover, inhibition by soybean trypsin inhibitor was eliminated for the modified trypsin. Kinetic analysis indicated that access of a high-molecular-weight substrate (casein) to the modified enzyme was restricted compared with a low-molecular-weight substrate.
- 社団法人日本農芸化学会の論文
- 1994-02-23
著者
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KOBAYASHI Mikihiko
National Food Research Institute
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Takatsu Koichiro
National Food Research Institute
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Sasaki Yoshihiro
National Food Research Institute
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- Characterization of Iron (II) : Comoplexes with β-Diketones and Monthio-β-diketone (Commemoration Issue Dedicated to Professor Tsunenobu Shigematsu on the Occasion of his Retirement)