Conversion of the Cleavage Specificity of Subtilisin YaB on Oxidized Insulin Chains to an Elastase-like Specificity by Replacement of Glyl24 with Ala(Biochemistry & Molecular Biology)

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概要

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• Replacement of Glyl24 on the S1 pocket of subtilisin YaB with Ala changed the cleavage pattern on oxidized insulin B-chain from the subtilisin type to the elastase type. The initial cleavage site in the B-chain shifted from L15-Y16 for wild-type YaB to A14-L15 for the G124A mutant. Upon complete hydrolysis with the G124A mutant, four of the six major cleavage sites on the B-chain were identical to porcine pancreatic elastase cleavage sites.

• 社団法人日本農芸化学会の論文
• 2003-07-23

著者

• TAKAGI Hiroshi

  Department of Surgery II, University School of Medicine

• Hsu C‐c

  Institute Of Biochemistry National Yang-ming University

• Tsai Y‐c

  National Yang‐ming Univ. Taipei Twn

• Takagi Hiroshi

  Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine

• Mei Hui-ching

  Institute Of Biochemistry National Yang-ming University:department Of Medical Technology China Medic

• Tsai Ying-Chieh

  Institute of Biochemistry, National Yang-Ming Medical College

• Tsai Ying-chieh

  Institute Of Biochemistry National Yang-ming Medical College

• LI Ywan-Feng

  Institute of Biochemistry, National Yang-Ming University

• HSU Chi-Cheng

  Institute of Biochemistry, National Yang-Ming University

• Li Ywan-feng

  Institute Of Biochemistry National Yang-ming University

• Takagi Hiroshi

  Department Of Bioscience Fukui Prefectural University

• Hsu Chi-cheng

  Institute Of Biochemistry National Yang-ming University

• Takagi Hiroshi

  Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University

• TAKAGI Hiroshi

  Department of Bioscience, Fukui Prefectural University

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