Production and Characterization of Keratinase of a Feather-degrading Bacillus licheniformis PWD-1
スポンサーリンク
概要
- 論文の詳細を見る
The keratinase produced by Bacillus licheniformis PWD-1 was induced by feather powder. Maximal enzyme production could be achieved by culturing in a medium containing 1% hammer-milled feather powder (100 mesh) at 45℃ for 30h. Maximal growth of PWD-1 was achieved at 50℃, and maximal enzyme induction was at 45℃. The molecular mass and isoelectric point of this enzyme were 31.4 kDa and 8.5, respectively. This enzyme was stable from pH 5 to 12. The optimal reaction pHs for feather powder and casein were 8.5 and 10.5 to 11.5, respectively. The optimal reaction temperature was 50℃ to 55℃. The relative activity of this enzyme toward casein, feather powder, keratin, elastin, and collagen was 1OO:52:41:18:7, and 100:56:32:3 for Suc-AAPL-pNA, Suc-AAPF-pNA, Suc-AAPM-pNA, and Suc-AAVA-pNA (Suc, succinyl ; pNA, p-nitrophenylanilide).
- 社団法人日本農芸化学会の論文
- 1995-12-23
著者
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Tsai Y‐c
National Yang‐ming Univ. Taipei Twn
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TAKAGI Hiroshi
Central Research Laboratories of Ajinomoto Co., Inc.
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Asano Minao
Central Research Laboratories Ajinomoto Co. Inc.
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Tsai Ying-Chieh
Institute of Biochemistry, National Yang-Ming Medical College
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Tsai Ying-chieh
Institute Of Biochemistry National Yang-ming Medical College
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Cheng Shu-Wen
Institute of Biochemistry, National Yang-Ming University
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Hu Hsien-Ming
Institute of Biochemistry, National Yang-Ming University
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Shen Shu-Whei
Institute of Biochemistry, National Yang-Ming University
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Shen Shu-whei
Institute Of Biochemistry National Yang-ming University
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Hu Hsien-ming
Institute Of Biochemistry National Yang-ming University
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Cheng Shu-wen
Institute Of Biochemistry National Yang-ming University
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Takagi Hiroshi
Central Research Laboratories Ajinomoto Co. Inc.
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