Versatile Cloning Vectors Constructed with Genes Indigenous to a Glutamic Acid-Producer, Brevibacterium lactofermentum(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
Versatile plasmid vectors useful for gene cloning in Brevibacterium lactofermentum, a glutamic acid-producing bacterium, have been constructed. The trimethoprim (Tp)-resistant dihydrofolate reductase gene derived from chromosomal DNA of the Tp-resistant mutant of B. lactofermentum was introduced into pAM330, a cryptic plasmid in B. lactofermentum. The constructed cloning vector pAJ228 (7.6kb) exists in 10 to 20 copies in cells of B. lactofermentum and donated Tp-resistance, which is a useful selective marker of transformants. pAJ228 was further improved to a versatile plasmid vector pAJ224 having some profitable characteristics such as smaller size (3.7 kb), higher copy number (60〜80 copies), and additional useful cloning sites (Bam HI, PstI and SalI) equipped with two different promoters arranged at both orientations for the expression of passenger DNA without promoter. These plasmids were stably retained in B. lactofermentum even in the absence of Tp over many generations. Thus, they have been found very powerful vectors for gene cloning in Brevibacterium and the related bacteria.
- 社団法人日本農芸化学会の論文
- 1986-10-23
著者
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Nakamori Shigeru
Central Research Laboratories of Ajinomoto Co., Inc.
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SANO Konosuke
Central Research Laboratories, Ajinomoto Co., Inc.
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Sano K
Ajinomoto Co. Inc. Kawasaki Jpn
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Sano Konosuke
Central Research Laboratories Of Ajinomoto Co. Inc.
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MORINAGA Yasushi
Institute of Life Sciences, Ajinomoto Co. , Inc.
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Sano Konosuke
Central Research Laboratories Ajinomoto Co. Inc.
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Matsui Kazuhiko
Central Research Laboratories Ajinomoto Co. Ltd.
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Nakamori Shigeru
Central Research Laboratories Ajinomoto Co. Inc.
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Takagi H
Nagoya Univ. Aichi Jpn
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Takagi H
Murata Manufacturing Co. Ltd. Kyoto Jpn
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MORINAGA YASUSHI
Central Research Laboratories of Ajinomoto Co., Inc.,
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MIWA Kiyoshi
Central Research Laboratories, Ajinomoto Co., Inc.
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TAKAGI Hiroshi
Central Research Laboratories of Ajinomoto Co., Inc.
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Miwa K
Ajinomoto Co. Inc. Kawasaki Jpn
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Miwa Kiyoshi
Central Research Laboratories Ajinomoto Co. Inc.
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Matsui Katsuhiko
Department Of Biological Chemistry School Of Pharmacy Hokuriku University
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Nakamori S
Ajinomoto Co. Inc. Kawasaki Jpn
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Morinaga Y
Institute Of Life Sciences Ajinomoto Co. Inc.
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Morinaga Yasushi
Central Research Laboratories Ajinomoto Co. Inc.
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Takagi Hiroshi
Central Research Laboratories Ajinomoto Co. Inc.
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