Altered Regulation Occurring in the Aromatic Amino Acid Biosynthetic Pathway of L-Tryptophan-producing Mutants Derived from Bacillus subtilis K
スポンサーリンク
概要
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The regulatory properties of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DS) and chorismate mutase (CM) were compared between L-Trp-producing mutants and the wild-type strain of Bacillus subtilis K. The synthesis of DS in the L-Trp producers was elevated 6- to 13-fold compared to that in the wild-type strain, however, its synthesis was still repressed when the mutants were cultured in the presence of L-Phe and/or L-Tyr. The DS from the L-Trp-producers was still sensitive to inhibition by chorismate and prephenate. The specific activity of the CM in the L-Trpproducers was only 3% of that in the case of the wild-type strain, which was due to the loss of activity of the CM 1-type enzyme and which contributed to the derepression of DS and the higher L-Trp accumulation.
- 社団法人 日本農芸化学会の論文
著者
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Enei Hitoshi
Central Research Laboratories Of Ajinomoto Co. Inc.
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Morinaga Yasushi
Central Research Laboratories Ajinomoto Co. Inc.
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Kurahashi Osamu
Central Research Laboratories Ajinomoto Co. Inc.
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Sato Katsuaki
Central Research Laboratories Ajinomoto Co. Inc.
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MORINAGA YASUSHI
Central Research Laboratories of Ajinomoto Co., Inc.
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NODA-WATANABE Mayumi
Central Research Laboratories of Ajinomoto Co., Inc.
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