Genetic Changes in Regulation Occurring in the Tryptophan Biosynthetic Pathway of L-Tryptophan Producing Mutants Derived from Bacillus subtilis K
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概要
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The regulatory mechanism for L-tryptophan (L-Trp) synthesis was compared between the wild type strain and L-Trp producing mutants of B. subtilis K. In the wild type strain, indolmycin (IM) repressed the synthesis of anthranilate synthetase (AS) more strongly than 5-fluorotryptophan (5FT), which repressed AS to the same extent as L-Trp did. 5FT inhibited the activity of AS as strongly as L-Trp did, while IM had no inhibitory effect. In the 5FT resistant strains, the syntheses of AS and tryptophan synthetase (TS-B) were markedly increased by genetic derepression, while AS remained still sensitive to the feedback inhibition by L-Trp. The facts that IM repressed the syntheses of AS and TS-B in the strain which was 5FTr and IMs, and did not repress those in the IM-resistant mutant suggested that IM acts as a co-repressor in a different way from 5FT.
- 社団法人 日本農芸化学会の論文
著者
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Enei Hitoshi
Central Research Laboratories Of Ajinomoto Co. Inc.
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Kurahashi Osamu
Central Research Laboratories of Ajinomoto Co., Inc.
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Morinaga Yasushi
Central Research Laboratories Ajinomoto Co. Inc.
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Kurahashi Osamu
Central Research Laboratories Ajinomoto Co. Inc.
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Sato Katsuaki
Central Research Laboratories Ajinomoto Co. Inc.
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NODA-WATANABE Mayumi
Central Research Laboratories of Ajinomoto Co., Inc.
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SATO Katsuaki
Central Research Laboratories of Ajinomoto Co., Inc.
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