Production of L-Phenylalanine by a Mutant of Brevibacterium lactofermentum 2256
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概要
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Excellent L-phenylalanine producers were screened from among tyrosine, methionine doubly auxotrophic and antimetabolite resistant mutants derived from a L-glutamic acid-producing bacterium; Brevibacterium lactofermentum 2256. A selected mutant, Brevibacterium lactofermentum No. 123, produced 21.7mg/ml of L-phenylalanine in 72 hr when 13% glucose was supplied as a carbon source. The accumulation of L-phenylalanine by strain No. 123 became 5.17-fold higher than that by parental strain No. 808. This yield was the highest among those so far reported. The addition of fumaric acid to the culture medium promoted the L-phenylalanine production. Accumulation of other amino acids in the culture broth was negligible compared with that of the main product, L-phenylalanine.
- 社団法人 日本農芸化学会の論文
著者
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Enei Hitoshi
Central Research Laboratories Of Ajinomoto Co. Inc.
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Morinaga Yasushi
Central Research Laboratories Ajinomoto Co. Inc.
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Yoshinaga Fumihiro
Central Research Laboratories Of Ajinomoto Co. Inc.
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Tsuchida Takayasu
Central Research Laboratories Of Ajinomoto Co. Inc.
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Kubota Koji
Central Research Laboratories Ajinomoto Co. Inc.
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Matsui Hiroshi
Central Research Laboratories Ajinomoto Co. Inc.
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