Effects of L-Serine Dehydratase Activity on L-Serine Production by Corynebacterium glycinophilum and an Examination of the Properities of the Enzyme

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概要

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• The results with Corynebacterium glycinophilum AJ-3170 and various mutants from AJ-3170 indicated that L-serine production was almost inversely proportional to L-serine degrading activity. The crude extract of the parental strain, AJ-3170,showed L-serine and L-threonine degrading activities. The 2 activities were completely separated from each other by gel-filtration, indicating that each activity comes from a different enzyme. The L-serine degrading enzyme, L-serine dehydratase (SD), was purified 30-fold from AJ-3170. Molecular weight of SD was 130,000. The enzyme was specific for L-serine, activated slightly by FeCl_2 and inhibited by MnCl_2. The double reciprocal plots of SD rate against substrate concentration gave an upwards-curved line. The value of [S]_<0.5> was 35 mM.

• 公益社団法人日本生物工学会の論文
• 1989-06-25

著者

• KUBOTA Koji

  Central Research Laboratories of Ajinomoto Co., Inc.

• Ozaki Hachiro

  Central Research Laboratories Ajinomoto Co. Inc.

• Ozaki Hachiro

  Central Research Laboratories Of Ajinomoto Co. Inc.

• Yokozeki Kenzo

  Central Research Laboratories Of Ajinomoto Co.

• Kubota Koji

  Central Research Laboratories Of Ajinomoto Co. Inc .

• Kubota Koji

  Central Research Laboratories Ajinomoto Co. Inc.

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