Gluco-Oligosaccharide and Galato-Oligosaccharide Production by Rhodotorula minuta IFO879
スポンサーリンク
概要
- 論文の詳細を見る
Three strains of yeast, Sterigmatomyces elviae CBS8119,Rhodotorula minuta IFO879 and Sirobasidium magnum CBS6803,which are know to produce galacto-oligosaccharide (Gals-OS) from lactose, were found to possess strong transglucosylation abilities and to produce gluco-oligosaccharide (Glc-OS) from celobiose. Among them, R. minuta IFO 879 was selected as the best producer of Glc-OS. Efficient production conditions for Gls-OS from cellobiose and Gal-OS from lactose were investigated. Using toluence-treated resting cells of R. minuta IFO879,the maximal amounts of Glc-OS produced from 200 mg/ml cellobiose and of Gal-OS produced from 200 mg/ml lactose were 70 mg/ml (a yield weight of 35%) and 76 mg/ml (a yield by weight of 38%), respectively. Since the by-product glucose was found to inhibit oligosaccharide production, it was removed from the reaction mixture by devising a suitable culture method such that the enzymatic reaction was accompanied by cell growth to consume the glucose. Under these conditions, the productivities were markedly improved : 201 mg/ml of Glc-OS was produced from 400 mg/ml cellobiose (a yield by weight of 51%) and 230 mg/ml of Gal-OS from 360 mg/ml of lactose (a yield by weight of 64%). The structures of the major components of Glc-OS and Gal-OS obtained by this method were identified as cellotriose and O-β-D-galactopyranosyl-(1→4)-O-β-D-galactopyranosyl-(1→4)-D-glucopyranose (4'-galactosyl-lactose), respectively.
- 社団法人日本生物工学会の論文
- 1996-08-25
著者
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YOKOZEKI Kenzo
Central Research Laboratories, Ajinomoto Co., Inc.,
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ONISHI Norimasa
Central Research Laboratories of Ajinomoto Co., Inc.
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Yokozeki Kenzo
Central Research Laboratories Of Ajinomoto Co.
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Yokozeki Kenzo
Central Research Laboratory Ajinomoto Co. Inc.
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Onishi N
Central Research Laboratory Ajinomoto Co. Inc.
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Onishi Norimasa
Central Research Laboratories Of Ajinomoto Co. Inc.
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