Enzymatic Production of 5-Methyluridine from Purine Nucleosides and Thymine by Erwinia carotovora AJ-2992(Microbiology & Fermentation Industry)

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概要

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• Microorganisms that produce 5-methyluridine (ribothymidine) directly from purine nucleosides and thymine were screened from our stock cultures. Of the 400 strains tested, Erwinia carotovora AJ-2992 was found to possess the most potent ability as to production of 5-methyluridine from guanosine and thymine. In the presence of intact cells of Er. carotovora AJ-2992 as the enzyme source, 222 m M 5-methyluridine was produced from 300mM guanosine and 300mM thymine at 60℃ on 48 hr incubation. The enzymatic production of 5-methyluridine by Er. carotovora AJ-2992 was found to involve the following two successive reactions via ribose-1-phosphate as an intermediate : phosphorolysis of purine nucleosides to ribose-1-phosphate and purine bases by purine nucleoside phosphorylase, followed by condensation of ribose-1-phosphate and thymine into 5-methyluridine by pyrimidine nucleoside phosphorylase.

• 社団法人日本農芸化学会の論文
• 1989-12-23

著者

• Yokozeki Kenzo

  Central Research Laboratories Of Ajinomoto Co.

• SHIRAE Hideyuki

  Central Research Laboratories of Ajinomoto Co., Inc.

• Shirae Hideyuki

  Central Research Laboratories Of Ajinomoto Co. Inc.

• Ishii Makoto

  Central Research Laboratories Mitsubishi Electric Corporation

• Ishii Makoto

  Central Research Laboratories Of Ajinomoto Co. Inc.

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