Purification and Characterization of Novel Transglutaminase from Bacillus subtilis Spores

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概要

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• Transglutaminase activity was detected in suspensions of purified spores prepared from lysozyme-treated sporulating cells of Bacillus subtilis AJ 1307. The enzyme was easily solubilized from the spores upon incubation at pH 10.5 at 37℃. The transglutaminase activity was separated into two fractions upon purification by hydrophobic interation chromatography (TG1 and TG2). Each enzyme was purified to electrophoretic homogeneity (about 1,000-fold). Both enzymes had the same molecular weight of 29,000 as estimated by SDSPAGE, had the same N-terminal 30 amino acid sequence, and also showed the same optimal temperature (60℃) and pH (8.2). The purified enzyme catalyzed formation of cross-linked ε-(γ-glutamyl) lysine isopeptides, resulting in the gel-formation of protein solutions such as α_s-casein and BSA.

• 社団法人日本農芸化学会の論文
• 2000-11-23

著者

• Suzuki S

  Department Of Chemistry Graduate School Of Science Osaka University

• KOBAYASHI Katsunori

  Central Research Laboratories, Ajinomoto Co., Inc.,

• YOKOZEKI Kenzo

  Central Research Laboratories, Ajinomoto Co., Inc.,

• YAMANAKA Shigeru

  Central Research Laboratories, Ajinomoto Co., Inc.,

• Suzuki S

  Aminoscience Laboratories Ajinomoto Co. Inc.

• Yokozeki K

  Ajinomoto. Co. Inc. Kawasaki Jpn

• Yokozeki Kenzo

  Aminoscience Laboratories Ajinomoto. Co. Inc.

• Eto Yuzuru

  Central Research Laboratories Ajinomoto Co. Inc.

• Suzuki Shunichi

  Central Research Laboratories Ajinomoto Co. Inc.

• Yamanaka Shigeru

  Central Research Laboratories Ajinomoto Co. Inc.

• Yamanaka Shigeru

  Faculty Of Txtile Science And Technology Shishu University

• Kobayashi Katsunori

  Central Research Laboratories Ajinomoto Co. Inc.

• KUBOTA Koji

  Central Research Laboratories of Ajinomoto Co., Inc.

• Kubota K

  Central Research Laboratories Ajinomoto Co. Inc.

• Kubota Koji

  The Research Institute Of Electrical Communication Tohoku University Presently The Technology Develo

• Kobayashi K

  Department Of Life Science (sports Sciences) The University Of Tokyo

• IZAWA Yuko

  Central Research Laboratories, Ajinomoto Co., Inc.

• Izawa Yuko

  Central Research Laboratories Ajinomoto Co. Inc.

• Yokozeki Kenzo

  Central Research Laboratories Of Ajinomoto Co.

• Yamanaka S

  Faculty Of Textile Science And Technology Shinshu University

• Yamanaka Shigeru

  Microbiology Group Institute Of Life Sciences Ajinomoto Co. Inc.:experimental Farm Faculty Of Textil

• Kubota K

  The Research Institute Of Electrical Communication Tohoku University Presently The Technology Develo

• Yamanaka Shigeru

  Basic Research Laboratories Central Research Laboratories Ajinomoto Co. Inc.

• Kubota Koji

  Central Research Laboratories Ajinomoto Co. Inc.

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