Reaction Mechanism of a New Glycosyltrehalose-hydrolyzing Enzyme Isolated from the Hyperthermophilic Archaeum, Sulfolobus solfataricus KM1
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概要
- 論文の詳細を見る
Amylolytic activity, which converts soluble starch to α,α-trehalose (trehalose), was found in the cell homogenate of the hyperthermophilic acidophilic archaeum, Sulfolobus solfataricus KM1. Two enzymes, a glycosyltransferase and an α-amylase, which were essential for this activity were identified. The α-amylase was purified to homogeneity on SDS-PAGE. The α-amylase catalyzed the hydrolysis of glycosyltrehaloses to trehalose. Analysis of the reaction products, kinetic parameters, and experimental findings using ^3H-labeled substrates indicated that the α-amylase hydrolyzed only the α-1,4 glucosidic linkage adjacent to the trehalose unit of the glycosyltrehaloses. Six strains of the Sufolobaceae family examined were observed to have the glycosyltrehalose-hydrolyzing enzyme, the αα-amylase.
- 社団法人日本農芸化学会の論文
- 1996-05-23
著者
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IWAMATSU Akihiro
Central Lab. Key Tech., Kirin Brewery Co.
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Miura Y
Department Of Applied Biological Science Tokyo Noko University
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Miura Yutaka
Applied Bioresearch Center Kirin Brewry Co. Ltd.
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Miura Yutaka
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Kato M
Nagoya Univ. Aichi Jpn
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Miyamoto Yoshiro
Department Of Biochemical Engineering Faculty Of Computer Science And System Engineering Kyushu Inst
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Iwamatsu A
Kirin Brewery Co. Ltd. Kanagawa Jpn
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Iwamatsu A
Kirin Brewery Co. Ltd. Yokohama Jpn
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Iwamatsu Akihiro
Central Lab. Key Tech. Kirin Brewery Co.
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KOBAYASHI Kazuo
Applied Bioresearch Center, Kirin Brewery Co., Ltd.
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KATO Masaru
Applied Bioresearch Center, Kirin Brewery Co., Ltd.
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KETTOKU Masako
Applied Bioresearch Center, Kirin Brewery Co., Ltd.
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KOMEDA Toshihiro
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd.
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Kobayashi K
Inst. Physical And Chemical Res.(riken) Saitama Jpn
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Kato M
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Kobayashi K
Department Of Life Science (sports Sciences) The University Of Tokyo
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Miura Y
Tokyo Noko Univ. Tokyo Jpn
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Komeda T
Central Laboratories For Key Technology Kirin Brewery Co. Ltd.
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Komeda Toshihiro
Central Laboratories For Key Technology Kirin Brewery Co. Ltd.
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Miura Yutaka
Dep. Of Applied Biological Sci. Tokyo Noko Univ.
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Miyamoto Yoko
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
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IWAMATSU Akihiro
Central Lab. Key Tech. , Kirin Brewery Co.
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