Purification and Characterization of New Trehalose-producing Enzymes Isolated from the Hyperthermophilic Archae, Sulfolobus solfataricus KM1

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概要

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• Amylolytic activity that converts soluble starch to α,α-trehalose (trehalose), was found in the cell homogenate of the hyperthermophilic acidophilic archae, Sulfolobus solfataricus KM1. DEAE chromatography of the homogenate as well as other new reliable assay methods showed two enzymes to be essential for this activity. These enzymes, a glycosyltransferase and an amylase, were purified to homogeneity and characterized. Their molecular masses were 76 kDa and 61 kDa and activities were maximal at 70-80℃ and 70-85℃, respectively. High thermostability was noted for each. The reaction products by the two enzymes on maltooligosaccharides were identified by ^1H- and ^<13>C-NMR spectra and HPLC analysis. The cooperative mechanism of the two enzymes was used in a new enzymatic pathway for trehalose synthesis from starch.

• 社団法人日本農芸化学会の論文
• 1996-03-23

著者

• SHINDO Kazutoshi

  Pharmaceutical Research Laboratory, Kirin Brewery Co. Ltd.

• IWAMATSU Akihiro

  Central Lab. Key Tech., Kirin Brewery Co.

• Miura Y

  Department Of Applied Biological Science Tokyo Noko University

• Miura Yutaka

  Applied Bioresearch Center Kirin Brewry Co. Ltd.

• Miura Yutaka

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Kato M

  Nagoya Univ. Aichi Jpn

• Miyamoto Yoshiro

  Department Of Biochemical Engineering Faculty Of Computer Science And System Engineering Kyushu Inst

• Iwamatsu A

  Kirin Brewery Co. Ltd. Kanagawa Jpn

• Iwamatsu A

  Kirin Brewery Co. Ltd. Yokohama Jpn

• Iwamatsu Akihiro

  Central Lab. Key Tech. Kirin Brewery Co.

• KOBAYASHI Kazuo

  Applied Bioresearch Center, Kirin Brewery Co., Ltd.

• KATO Masaru

  Applied Bioresearch Center, Kirin Brewery Co., Ltd.

• KETTOKU Masako

  Applied Bioresearch Center, Kirin Brewery Co., Ltd.

• Kobayashi K

  Inst. Physical And Chemical Res.(riken) Saitama Jpn

• Shindo Kazutoshi

  Pharmaceutical Research Laboratories Kirin Brewery Co. Ltd.

• Kato M

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Kobayashi K

  Department Of Life Science (sports Sciences) The University Of Tokyo

• Shindo K

  Department Of Food And Nutrition Japan Women's University

• Miura Y

  Tokyo Noko Univ. Tokyo Jpn

• Miura Yutaka

  Dep. Of Applied Biological Sci. Tokyo Noko Univ.

• Shindo Kazutoshi

  Japan Women's University

• Miyamoto Yoko

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• IWAMATSU Akihiro

  Central Lab. Key Tech. , Kirin Brewery Co.

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