Mode of AmyR Binding to the CGGN_8AGG Sequence in the Aspergillus oryzae taaG2 Promoter
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概要
- 論文の詳細を見る
AmyR is a transcriptional activator in Aspergillus spp. necessary for induction of the amylolytic enzyme genes. It recognizes 5′-CGGN8CGG-3′ conserved in a number of the amylolytic gene promoters, and in addition 5′-CGGAAATTTAA-3′ in the A. oryzae α-amylase promoter. In this report, interaction of AmyR with the 5′-CGGAAATTTAA-3′ type binding site in the Taka-amylase gene (taaG2) promoter was precisely characterized by DNase I footprinting analysis and electrophoretic mobility shift assay in vitro, and also by examination of the in vivo activity of the mutated promoters. The in vitro and in vivo analyses indicated that two AmyR molecules bind cooperatively to the 5′-CGGAAATTTAA-3′ sequence by recognizing the CGG triplet at the 5′-end and the AGG triplet just downstream of the sequence.
- 社団法人 日本農芸化学会の論文
- 2004-09-23
著者
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KOBAYASHI Tetsuo
Department of Anatomy II School of Veterinary Medicine, Azabu University
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KATO Masashi
Department of Engineering Physics, Electronics and Mechanics, Nagoya Institute of Technology
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ITO Tatsuo
Department of Pediatrics, Hirosaki University School of Medicine
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Itoh Tomoyuki
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Kato M
Nagoya Univ. Aichi Jpn
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TSUKAGOSHI Norihiro
Department of Food Science and Technology, Faculty of Agriculture, Nagoya University
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Itoh T
Laboratory Of Animal Food Science College Of Bioresource Science Nihon University
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Kobayashi Tetsuo
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Kato M
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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TANI Shuji
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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Kobayashi T
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Itoh T
Faculty Of Agriculture Iwate University
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Itoh T
Hiroshima Univ. Higashi-hiroshima Jpn
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Kobayashi T
Laboratory Of Microbiology The Institute Of Physical And Chemical Research (riken)
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Kato Masashi
Department Of Bioenvironmental Medicine Graduate School Of Medicine Chiba University
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Tani Shuji
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Tani Shuji
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Kobayashi T
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Tsukagoshi N
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Tsukagoshi Norihiro
Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University
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Kobayashi Tetsuo
Department Of Anatomy Ii School Of Veterinary Medicine Azabu University
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Ito Tatsuo
Department Of Pediatrics Hirosaki University School Of Medicine
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Kato Masashi
Department Of Applied Biological Chemistry Fuculty Of Agriculture Meijo University
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Kato Masashi
Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University
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Ito Tatsuo
Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University
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KOBAYASHI Tetsuo
Department of Anatomy II
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