Effects of Maternal Exposure to Low Doses of DES on Testicular Steroidogenesis and Spermatogenesis in Male Rat Offspring
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概要
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Our previous studies have demonstrated that prenatally administered diethylstilbestrol (DES) impairs testicular endocrine function in male offspring. The present study examined whether maternal DES treatment influences testicular steroidogenesis and spermatogenesis. DES was injected subcutaneously at 0.5 or 1.5 μg/kg/day (DES 0.5 and 1.5 groups, respectively) into pregnant SD rats on days 7-21 of gestation. Male offspring in the DES 0.5 and 1.5 groups were autopsied at 1, 3, 6 and 15 weeks after birth. At 1 week, DES treatment did not lead to a change in the volume of P450scc-positive cells (Leydig cells), suggesting that DES has no inhibitory effect on the development of Leydig cells. DES administration disrupted luteinizing hormone receptor (LHr) expression and exerted inhibitory effects on signal transduction from LHr to steroidogenic acute regulatory protein (StAR) in testicular steroidogenesis (P<0.05), although there were no changes in the mRNA expression levels of steroidogenic enzymes, such as P450scc, 3β-hydroxysteroid dehydrogenase (3β-HSD) and P45017 α, which may have caused a decrease in the plasma testosterone level. DES treatment did not disrupt the cycle of spermatogenesis but did upregulate the expression levels of androgen receptor (AR) mRNA in both DES groups at 15 weeks (P<0.05). These results indicate that maternal DES treatment disrupts steroidogenesis but induces a high level of AR mRNA expression to counteract the low levels of testosterone during spermatogenesis.
- 2009-12-01
著者
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Murakami Masaru
Department of Obstetrics and Gynecology, Tokai University School of Medicine
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KOBAYASHI Tetsuo
Department of Anatomy II School of Veterinary Medicine, Azabu University
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SHIRAI Mitsuyuki
Department of Veterinary Pharmacology School of Veterinary Medicine, Azabu University
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SAKAUE Motoharu
Department of Anatomy II School of Veterinary Medicine, Azabu University
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OCHIAI Hideharu
Research Institute of Biosciences, Azabu University
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ARISHIMA Kazuyoshi
Department of Anatomy II School of Veterinary Medicine, Azabu University
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YAMAMOTO Masako
Department of Anatomy II School of Veterinary Medicine, Azabu University
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Sakaue Motoharu
Department Of Anatomy Ii School Of Veterinary Medicine Azabu University
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Yamamoto Masako
Department Of Anatomy Ii School Of Veterinary Medicine Azabu University
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Ochiai Hideharu
Research Institute Of Biosciences Azabu University
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Shirai Mitsuyuki
School Of Veterinary Medicine Azabu University
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Shirai Mitsuyuki
Department Of Veterinary Pharmacology School Of Veterinary Medicine Azabu University
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Arishima Kazuyoshi
Department Of Anatomy Ii School Of Veterinary Medicine Azabu University
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Arishima Kazuyoshi
Department Of Veterinary Anatomy Ii Azabu University School Of Veterinary Medicine
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Arishima Kazuyoshi
Anatomy Ii School Of Veterinary Medicine Azabu University
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Arishima Kazuyoshi
Department Of Anatomy Ii Azabu University School Of Veterinary Medicine
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Yamamoto Masaru
Laboratories Of Natural Information Science Graduate School Of Integrated Science Yokohama City Univ
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Murakami Masaru
Department Of Molecular Biology School Of Veterinary Medicine Azabu University
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Yamamoto Masaru
Laboratories Of Natural Information Science Graduate School Of Integrated Science Yokohama City Univ
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Yamamoto Masako
Department Of Anatomy Ii Azabu University School Of Veterinary Medicine
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Kobayashi Tetsuo
Department Of Anatomy Ii School Of Veterinary Medicine Azabu University
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Murakami Masaru
Department Of Cardiology Tokushima Prefectural Central Hospital
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Ochiai Hideharu
Research Institute Of Biosciences
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Yamamoto Masahiro
Center For Kampo Medicine Keio University School Of Medicine
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ARISHIMA Kazuyoshi
Department of Anatomy II
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SHIRAI Mitsuyuki
Department of Pharmacology, Azabu University School of Veterinary Medicine
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KOBAYASHI Tetsuo
Department of Anatomy II
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