Comparison of K-Cl Cotransport Expression in High and Low K Dog Erythrocytes

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概要

• 論文の詳細を見る

• K-Cl cotransport plays a crucial role in regulatory volume decrease of erythrocytes. K-Cl cotransport activities in dog erythrocytes with an inherited high Na-K pump activity (HK) and normal erythrocytes (LK) were compared. Nitrite (NO2) stimulated K-Cl cotransport activity in HK cells around 14-fold at 2.4 mM, and it also increased the Km value of this cotransporter. Real-time PCR and western blot analysis revealed that K-Cl cotransporter 1 was dominant, and that the quantity of K-Cl cotransporter 1 protein was comparable between HK and LK erythrocytes. These results suggest that the difference in cotransport activity was not caused by the amount of K-Cl cotransport protein but by a difference in the regulation system, which is susceptible to oxidant.

• 社団法人　日本実験動物学会の論文
• 2006-01-01

著者

• OCHIAI Hideharu

  Research Institute of Biosciences, Azabu University

• Ochiai Hideharu

  Institute Of Biosciences School Of Veterinary Medicine Azabu University

• Ochiai Hideharu

  Research Institute Of Biosciences Azabu University

• Fujise H

  School Of Veterinary Medicine Azabu University

• Fujise Hiroshi

  Department Of Veterinary Pathology Ii School Of Veterinary Medicine Azabu University

• HISAMATSU Shin

  Laboratory of Environmental Chemistry, Graduate School of Environmental Health, Azabu University

• HIGA Kazunari

  Laboratory of Pathobiochemistry, School of Veterinary Medicine, College of Environmental Health, Aza

• FUJISE Hiroshi

  Research Institute of Biosciences, College of Environmental Health, Azabu University

• Hisamatsu Shin

  Laboratory Of Environmental Chemistry College Of Environmental Health Azabu University

• Higa Kazunari

  Laboratory Of Pathobiochemistry School Of Veterinary Medicine Azabu University

• Ochiai Hideharu

  Research Institute Of Biosciences

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