Comparison of Glycerol, Lactamide, Acetamide and Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit Spermatozoa
スポンサーリンク
概要
- 論文の詳細を見る
The rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese white rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 ± 3.0%) was significantly (P<0.05) higher than in glycerol (17.0 ± 3.3%). In addition, the rates of sperm plasma membrane integrity in lactamide and acetamide (35.9 ± 3.3% and 30.2 ± 3.0%, respectively) were significantly (P<0.05) higher than in glycerol (17.0 ± 2.6%). The results indicate that 1.0 M lactamide and acetamide have higher cryoprotective effects than 1.0 M glycerol for cryopreservation of Japanese white rabbit spermatozoa.
- 日本繁殖生物学会の論文
- 2006-08-01
著者
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SEITA Yasunari
Laboratory of Animal Reproduction, Graduate School of Veterinary Science, Azabu University
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KASHIWAZAKI Naomi
Laboratory of Animal Reproduction, Graduate School of Veterinary Science, Azabu University
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Seita Yasunari
Laboratory Of Animal Reproduction Azabu University
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Seita Yasunari
Laboratory Of Animal Reproduction Graduate School Of Veterinary Science Azabu University
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Masaoka Toshio
Laboratory Of Comparative Toxicology School Of Veterinary Medicine Azabu University
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SHINO Masao
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
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Kashiwazaki Naomi
Laboratory Of Animal Reproduction Azabu University
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Kashiwazaki N
School Of Veterinary Medicine Azabu University
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Kashiwazaki Naomi
Laboratory Of Animal Reproduction Graduate School Of Veterinary Science Azabu University
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Shino Masao
Laboratory Of Animal Reproduction School Of Veterinary Medicine Azabu University
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Inomata Tomo
Laboratory Of Experimental Animal Science Graduate School Of Veterinary Science Azabu University
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OKUDA Yasushi
Laboraory of Animal Reproduction, Graduate School of Veterinary Medicine, Azabu University
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HISAMATSU Shin
Laboratory of Environmental Chemistry, Graduate School of Environmental Health, Azabu University
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SONOKI Shigenori
Laboratory of Environmental Chemistry, Faculty of Environment and Health, Azabu University
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Okuda Yasushi
Laboratory Of Animal Reproduction School Of Veterinary Medicine Azabu University
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Hisamatsu Shin
Laboratory Of Environmental Chemistry College Of Environmental Health Azabu University
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Shino Masao
Laboratory Of Animal Reproduction Department Of Animal Science And Biotechnology School Of Veterinar
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Hisamatsu S
College Of Environmental Health Azabu University
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Sonoki S
Laboratory Of Environmental Chemistry Faculty Of Environment And Health Azabu University
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SHINO Masao
Laboraory of Animal Reproduction, Graduate School of Veterinary Medicine, Azabu University
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KASHIWAZAKI Naomi
Laboraory of Animal Reproduction, Graduate School of Veterinary Medicine, Azabu University
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INOMATA Tomo
Laboraory of Animal Reproduction, Graduate School of Veterinary Medicine, Azabu University
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