Effect of Centrifugation Treatment before Vitrification on the Viability of Porcine Mature Oocytes and Zygotes Produced In Vitro

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概要

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• The aim of the present study was to investigate the effects of centrifugation pretreatment on the viability and nuclear status of porcine in vitro matured (IVM) oocytes and on the developmental competence of in vitro fertilized (IVF) oocytes (zygotes) after cryopreservation by vitrification (Solid Surface Vitrification; SSV). Mature oocytes having the first polar body after IVM and zygotes having the second polar body at 10 h after IVF were centrifuged at 10,000 × g at 37 C for 20 min and then subjected to SSV. Their viability was evaluated by morphological appearance and fluorescein diacetate staining. The nuclear status of oocytes was evaluated 6 h after vitrification. The developmental ability to the blastocyst stage of vitrified zygotes was evaluated after 6 days of in vitro culture. Although centrifugation did not damage the oocytes directly, it drastically reduced the rate of live oocytes after SSV. The rates of vitrification-induced parthenogenetic activation were similar in both centrifuged and non-centrifuged oocytes (42.4 and 47.4%, respectively). Centrifugation had no significant effects on the viability of pronuclear oocytes. The development of vitrified zygotes to the blastocyst stage was significantly lower than that of the control irrespective of centrifugation pretreatment. There was no difference in the cleavage and blastocyst rates between the control and centrifuged zygotes after vitrification. There was also no difference in the total cell numbers of blastocysts between the control and centrifuged zygotes irrespective of vitrification. These results reveal that, in IVM porcine oocytes, centrifugation pretreatment is highly detrimental to cryotolerance; however, in zygotes, it has only a slight effect on viability and does not alter the developmental competence of surviving zygotes.

• 日本繁殖生物学会の論文
• 2008-06-01

著者

• Somfai Tamas

  National Livestock Breeding Center

• NOGUCHI Junko

  Division of Agrobiological Resource II, National Institute of Agrobiological Resource

• Kikuchi Kazuhiro

  東京農工大学 大学院農学府

• Kikuchi Kazuhiro

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• NAGAI Takashi

  National Institute of Livestock and Grassland Science

• Kikuchi Kazuhiro

  Dep. Of Animal Sci. National Inst. Of Agrobiological Sciences

• 永井 卓

  (独)・農業・生物系特定産業技術研究機構・畜産草地研究所

• Somfai Tamas

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• Ozawa Manabu

  Department Of Animal Science National Institute Of Agrobiological Sciences

• Ozawa Manabu

  Institute Of Agriculture And Forestry University Of Tsukuba

• Kikuchi Kazuhiro

  Department Of Animal Science National Institute Of Agrobiological Sciences

• Nagai Takashi

  Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science

• NAKAI Michiko

  Division of Animal Sciences, National Institute of Agrobiological Sciences

• KANEKO Hiroyuki

  Division of Animal Sciences, National Institute of Agrobiological Sciences

• KASHIWAZAKI Naomi

  Graduate School, Azabu University

• OZAWA Manabu

  Division of Animal Sciences, National Institute of Agrobiological Sciences

• MAEDOMARI Naoki

  Graduate School, Azabu University

• Kaneko Hiroyuki

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• Kaneko H

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• Kashiwazaki Naomi

  Laboratory Of Animal Reproduction Azabu University

• Kashiwazaki N

  School Of Veterinary Medicine Azabu University

• Kashiwazaki Naomi

  Laboratory Of Animal Reproduction Graduate School Of Veterinary Science Azabu University

• Kashiwazaki Naomi

  Graduate School Of Veterinary Medicine Azabu University

• Noguchi J

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• Noguchi Junko

  Positron Medical Center Tokyo Metropolitan Institute Of Gerontology

• Ozawa Manabu

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• Maedomari Naoki

  Graduate School Azabu University

• Nagai T

  Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science

• Nagai Takuya

  Nasu Institute Nisshin Flour Milling Co. Ltd.

• Nagai Takashi

  National Inst. Of Livestock And Grassland Sci.

• Nakai Michiko

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• NAKAI Michiko

  Division of Animal Science, National Institute of Agrobiological Sciences

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