The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos
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概要
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The aim of this study was to investigate if reconstructed felid embryos obtained by intraspecies or intergeneric cloning can develop in vitro. Fibroblast cells (f) from a domestic cat (DCf), marbled cat (MCf) and bovine (Bf) were used as donor cells, and oocytes (o) from domestic cats (DCo) and bovine (Bo) were used as recipient cytoplasts. There were two intraspecies (donor cell + recipient cytoplast: DCf + DCo and Bf + Bo) and three intergeneric (MCf + DCo, DCf + Bo and MCf + Bo) cloning groups in the study. In Experiment 1, the effects of manipulation media, modified TCM-199 (199H) or Emcare holding medium (EHM), on in vitro development of DCf + DCo embryos were investigated. The blastocyst formation rate (BFR) of the embryos manipulated in EHM (33.3%) was higher (P<0.05) compared with those manipulated in 199H (18.1%). In Experiment 2, DCf + DCo and MCf + DCo embryos were cocultured with or without domestic cat oviductal epithelium cells. Irrespective of coculture, the same BFR was obtained for DCf + DCo embryos (44.4 vs. 38.0%), while MCf + DCo embryos could not develop beyond the morula stage. In experiment 3, although the development of MCf + DCo and DCf + Bo embryos was arrested at the morula stage, 8.6% of MCf + Bo embryos were able to develop to the blastocyst stage. These results demonstrated that EHM was superior to 199H as an embryo manipulation medium and that the DCo and Bo could support the early embryonic development of intergeneric cloned marbled cat embryos up to the morula stage. However, postimplantation development still needs to be investigated.
著者
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NAGAI Takashi
National Institute of Livestock and Grassland Science
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IMSOONTHORNRUKSA Sumeth
Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te
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LORTHONGPANICH Chanchao
Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te
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SANGMALEE Anawat
Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te
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SRIRATTANA Kanokwan
Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te
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LAOWTAMMATHRON Chuti
Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te
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TUNWATTANA Wanchai
Chiangmai Zoo
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SOMSA Wachiravit
Nakhon Ratchasima Zoo
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KETUDAT-CAIRNS Mariena
Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te
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PARNPAI Rangsun
Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te
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Ketudat-cairns Mariena
Embryo Technol. And Stem Cell Res. Center School Of Biotechnology Suranaree Univ. Of Technol.
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Parnpai Rangsun
Embryo Technol. And Stem Cell Res. Center School Of Biotechnology Suranaree Univ. Of Technol.
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Nagai Takashi
National Inst. Of Livestock And Grassland Sci.
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Srirattana Kanokwan
Embryo Technol. And Stem Cell Res. Center School Of Biotechnology Suranaree Univ. Of Technol.
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Laowtammathron Chuti
Embryo Technol. And Stem Cell Res. Center School Of Biotechnology Suranaree Univ. Of Technol.
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Sangmalee Anawat
Embryo Technology And Stem Cell Research Center School Of Biotechnology Suranaree University Of Tech
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Imsoonthornruksa Sumeth
Embryo Technology And Stem Cell Research Center School Of Biotechnology Suranaree University Of Tech
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