Vitrification of Fully Grown and Growing Porcine Oocytes Using Germinal Vesicle Transfer
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概要
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The survival rate of vitrified germinal vesicle (GV) stage porcine oocytes is very low, and it is not known if the vitrification damages the nucleus, cytoplasm or both. We have evaluated the eventual GV or cytoplasmic damage in fully grown (FG) and growing vitrified oocytes. Fifty-five percent of nonvitrified FG cumulus-denuded oocytes reached the metaphase II (MII) stage in culture. When growing oocytes from preantral (PA) and early antral (EA) follicles were matured in vitro, almost all oocytes were arrested at the GV stage (GV stage: PA 88.9 and EA 79.5%, respectively). When fresh GVs from FG, PA and EA oocytes were transferred into fresh enucleated FG oocytes and matured in vitro, some of them reached the MII stage (MII stage: FG/FG 57.5%, PA/FG 9.3% and EA/FG 35.3%, respectively). The maturation rate of vitrified FG oocytes was only 6.1% but increased dramatically when vitrified GVs from FG, PA and EA oocytes were transferred into fresh enucleated FG oocytes (MII stage: VitFG/FG 43.9%, VitPA/FG 7.1% and VitEA/FG 26.3%, respectively). These results were not significantly different from those for the nonvitrified groups (MII stage: FG/FG 57.5%, PA/FG 9.3% and EA/FG 35.3%, respectively). We activated the reconstructed oocytes that received fresh or vitrified GVs (FG/FG, EA/FG, VitFG/FG and VitEA/FG) and examined their embryonic development. Cleaved embryos (nonvitrified groups 13.0-61.8%, vitrified groups 33.3-40.0%) and blastocysts (nonvitrified groups 0.0-18.2%, vitrified groups 0.0-2.9%) were obtained after activation. These results demonstrate that vitrified porcine GVs maintain maturational and developmental competence and that vitrification predominantly damages the cytoplasm.
著者
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MANABE Noboru
Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of
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Kikuchi Kazuhiro
東京農工大学 大学院農学府
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Kikuchi Kazuhiro
Division Of Animal Sciences National Institute Of Agrobiological Sciences
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NAGAI Takashi
National Institute of Livestock and Grassland Science
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Kikuchi Kazuhiro
Dep. Of Animal Sci. National Inst. Of Agrobiological Sciences
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NAKAGAWA Shoma
Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of
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MAEDOMARI Naoki
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
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MIYANO Takashi
Graduate School of Agricultural Science, Kobe University
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FULKA JR
Institute of Animal Science
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Fulka Jr
Institute Of Animal Production
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Manabe Noboru
Animal Resource Center The University Of Tokyo:research Center. For Food Safety The University Of To
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Maedomari Naoki
Graduate School Azabu University
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Miyano Takashi
Graduate School Of Agricultural Sci. Kobe Univ.
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Nagai Takashi
National Inst. Of Livestock And Grassland Sci.
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