Effect of Volume of Non-Surgical Embryo Transfer Medium on Ability of Porcine Embryos to Survive to Term
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概要
- 論文の詳細を見る
Non-surgical embryo transfer is a promising method for improving efficiency in the pork industry and also for biotechnology applications, such as in vitro embryo production, transgenesis and cloning. Several groups have reported successful piglet production using an artificial insemination catheter or flexible catheter designed for this procedure; however, the efficiency of the technique is still low. The critical points that need to be addressed in order to improve this procedure are (1) the embryo deposition site and (2) volume of transfer medium associated with the embryos; however, the latter has not yet been examined systematically. In the present study, we evaluated the effect of the volume of non-surgical embryo transfer medium on the ability of porcine embryos to survive to term by using a recently produced flexible catheter. The catheter consists of a guide and an injector. Blastocysts 200-230 μm in diameter were collected from donor gilts and transferred to recipient gilts. The time required for the completion of embryo transfer using this catheter was 14.6 ± 3.9 min. The tip of the injector was determined by laparotomy to be located in a uterine horn 20-30 cm anterior from the branching point of the uterus body. We transferred 17.0-17.3 embryos with different volumes of medium (1.6, 3.2 and 10 ml) into each of 5, 4 and 4 recipients, respectively, and pregnancy was confirmed in 4, 3 and 1 of these recipients, respectively. Three recipients in the 1.6 ml group farrowed a total of 19 piglets (4, 5 and 10 piglets, respectively). These results suggest that successful non-surgical embryo transfer is affected by the volume of transfer medium.
- 日本繁殖生物学会の論文
- 2008-02-01
著者
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Tajima Shigeyuki
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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Kikuchi Kazuhiro
Division Of Animal Sciences National Institute Of Agrobiological Sciences
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NAKAZAWA Yoshinori
Kanagawa Prefectural Livestock Industry Technology Center
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Ueda Jun-ichi
Department Of Bioregulation Research National Institute Of Radiological Sciences
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Ueda Jun-ichi
Department Of Animal Industry Aichi Agricultural Research Center
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MISAWA Hiroyasu
Asahidai Factory, Misawa Medical Industry Co., Ltd.
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FUJINO Yukihiro
Livestock Research Center, Saitama Prefectural Agriculture and Forestry Research Center
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MISUMI Koji
Department of Technology, National Livestock Breeding Center
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NAKAMURA Yoshiyuki
Livestock Research Center, Saitama Prefectural Agriculture and Forestry Research Center
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SHIBATA Takako
Department of Animal Industry, Aichi Agricultural Research Center
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HIRAYAMA Yuri
Department of Technology, National Livestock Breeding Center
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Tajima Shigeyuki
Department Of Agricultural Chemistry Faculty Of Agriculture Nagoya University
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Tajima Shigeyuki
Department Of Animal Industry Aichi Agricultural Research Center
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Misumi Koji
Department Of Technology National Livestock Breeding Center
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Hirayama Yuri
Department Of Technology National Livestock Breeding Center
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Shibata Takako
Department Of Animal Industry Aichi Agricultural Research Center
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Misawa Hiroyasu
Asahidai Factory Misawa Medical Industry Co. Ltd.
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Fujino Yukihiro
Livestock Research Center Saitama Prefectural Agriculture And Forestry Research Center
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Nakamura Yoshiyuki
Livestock Research Center Saitama Prefectural Agriculture And Forestry Research Center
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