Purification and Characterization of an Endo-1, 4-β-D-Galactanase from Aspergillus sojae
スポンサーリンク
概要
- 論文の詳細を見る
An endo-1, 4-β-D-galactanase(EC 3.2.1.89)was purified to homogeneity from a solid-state culture of Aspergillus sojae. The molecular weight of the galactanase was estimated to be 39, 700 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE). Gel filtration chromatography indicated the native enzyme to be a monomer. The isoelectric point of the galactanase was 3.60. The optimum pH and temperature of the enzyme activity were 4.5 and 50℃, respectively. The galactanase was stable from pH 6.0 to 10.0, and up to 35℃. The K_m value for arabinogalactan from soybean was 0.82 mg/ml. The activity of the enzyme was significantly inhibited by Mn^<2+> Hg^<2+> Ag^+, and Fe^<3+> and no stimulation by metal ions was apparent. After the hydrolysis of arabinogalactan from soybean, the major products were galactobiose and galactose, and no liberation of arabinose was observed in the reaction mixture.
- 社団法人日本生物工学会の論文
- 1998-01-25
著者
-
Tajima Shigeyuki
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
-
Tajima Shigeyuki
Kagawa Univ. Kagawa Jpn
-
Yoshioka Naomi
Kagawa Prefectural Fermentation And Food Experimental Station
-
KIMURA Isao
Kagawa Prefectural Industrial Center
-
Tajima S
Department Of Life Science Faculty Of Agriculture Kagawa University
-
Tajima Shigeyuki
Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University
-
Tajima Shigeyuki
Department Of Agricultural Chemistry Faculty Of Agriculture Nagoya University
-
Kimura I
Kagawa Prefectural Fermentation And Food Experimental Station
-
Kimura Isao
Kagawa Prefectural Fermentation And Food Experimental Station
関連論文
- Purification and Characterization of D-Tagatose 3-Epimerase from Pseudomonas sp. ST-24
- The 5'-end expressed sequence tags of Lotus japonicus
- 8-20 Screening of Mesorhizobium loti mutants for symbiotic N_2 fixation in Lotus japonicus
- Identification of a Sed5-like SNARE Gene LjSYP32-1 that Contributes to Nodule Tissue Formation of Lotus japonicus
- Differential Expression Pattern of C_4 Bundle Sheath Expression Genes in Rice, a C_3 Plant
- The Promoter for C_4-type Mitochondrial Aspartate Aminotransferase Does not Direct Bundle Sheath-specific Expression in Transgenic Rice Plants
- EXPRESSION OF ASPARTATE AMINOTRANSFERASE GENE OF C4 PANICUM MILIACEUMIN RICE
- THE PROMOTER OF rbcS IN A C3 PLANT (RICE) DIRECTS ORGAN-SPECIFIC, LIGHT-DEPENDENT EXPRESSION IN A C4 PLANT (MAIZE), BUT DOES NOT CONFER THE BUNDLE SHEATH CELL-SPECIFIC EXPRESSION
- FUNCTION ANALYSIS OF PYRUVATE Pi DIKINASE IN RICE
- URICASE GENE EXPRESSION IN A NON-ALLANTOIN TYPE LEGUME, MEDICAGO SATIVA
- HOW HAD THE C4-type PDK GENE ESTABLISHED DURING THE EVOLUTION FROM C3- TO C4-PLANT?
- EVOLUTION AND EXPRESSION OF the Pdk GENE IN RICE
- Global Gene Expression in Bradyrhizobium japonicum Cultured with Vanillin, Vanillate, 4-Hydroxybenzoate and Protocatechuate
- NAD^+-malic enzyme affects nitrogenase activity of Mesorhizobium loti bacteroids in Lotus japonicus nodules
- Effect of Volume of Non-Surgical Embryo Transfer Medium on Ability of Porcine Embryos to Survive to Term
- Differential protein profiles of Bradyrhizobium japonicum USDA110 bacteroid during soybean nodule development(Soil Biology)
- Host plant genome overcomes the lack of a bacterial gene for symbiotic nitrogen fixation
- 8-23 Novel metallochaperone in the assembly of cytochrome c oxidase of soybean nodule bacteroids
- NAD-Malic Enzyme Affects Nitrogen Fixing Activity of Bradyrhizobium japonicum USDA 110 Bacteroids in Soybean Nodules
- 8-11 Proteome analysis of Bradyrhizobium japonicum USDA110 bacteroid differentiation during soybean nodule development
- Genetic Diversity of Native Bradyrhizobium Populations in Soybean-Growing Areas of Northern Thailand (Soil Biology)
- Symbiotic Efficiency and Compatibility of Native Rhizobia in Northern Thailand with Different Soybean Cultivars III. Laboratory Experiment Using Native Isolates from Upland Rainfed Soybean-Growing Areas
- Symbiotic Efficiency and Compatibility of Native Rhizobia in Northern Thailand with Different Soybean Cultivars II. Laboratory Experiment Using Native Isolates from Irrigated Soybean-Growing Areas
- Symbiotic Efficiency and Compatibility of Native Rhizobia in Northern Thailand with Different Soybean Cultivars I. Field Experiment in Irrigated Traditional Soybean-Growing Area
- Expression Cloning of a Variety of Novel Protein Kinases in Lotus japonicus
- Characterization of Local Rhizobia in Thailand and Distribution of Malic Enzymes
- Cloning and Characterization of a NADP^+ -Malic Enzyme Gene from Bradyrhizobium japonicum USDA110
- Identification and Typing of Miso and Soy Sauce Fermentation Yeasts, Candida etchellsii and C. versatilis, Based on Sequence Analyses of the D1D2 Domain of the 26S Ribosomal RNA Gene, and the Region of Internal Transcribed Spacer 1, 5.8S Ribosomal RNA Gen
- Soybean Cotyledons Contain a Uricase That Cross-Reacts with Antibodies Raised against the Nodule Uricase (Nod-35)
- Purification and Properties of an alpha-L-Arabinofuranosidase from Cotyledons of Soybean Seedlings(Biological Chemistry)
- Cloning, Sequencing and Expression of an α-L-Arabinofuranosidase from Aspergillus sojae
- Purification and Characterization of a β-Glucosidase with β-Xylosidase Activity from Aspergillus sojae
- Purification and Characterization of an Endo-1, 4-β-D-Galactanase from Aspergillus sojae
- Succinate Degradation through the Citric Acid Cycle in Bradyrhizobium japonicum J501 Bacteroids under Low Oxygen Concentrations(Biological Chemistry)
- Proteomic Analysis on Symbiotic Differentiation of Mitochondria in Soybean Nodules
- Characterization of Bacteroid Proteins in Soybean Nodules Formed with Bradyrhizobium japonicum USDA110
- Regulation of uricase activity in developing roots of Glycine max, non-nodulating variety A62-2
- Cadaverine Involved in Urate Degrading Activity (Uricase Activity) in Soybean Radicles
- Molecular Analysis of Ribosomal RNA Gene of Red Tide Algae Obtained from the Seto Inland Sea
- Application of Repetitive Extragenic Palindromic (REP)-PCR and Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR Analysis to the Identification and Classification of Japan and Thai Local Isolates of Bradyrhizobium japonicum, Shinorhizobium melilo
- A molecular phylogenic analysis of marine red tide microalgae from the Seto Inland Sea
- A molecular phylogenic analysis of marine red tide phytoplankton
- Characteristics of a Urate-Degrading Diamine Oxidase-Peroxidase Enzyme System in Soybean Radicles
- Nucleotide Pools in Soybean Nodule Tissues, a Survey of NAD(P)/NAD(P)H Ratios and Energy Charge
- LjnsRING, a Novel RING Finger Protein, is Required for Symbiotic Interactions Between Mesorhizobium loti and Lotus japonicus
- Phosphoenolpyruvate Carboxylase Plays a Crucial Role in Limiting Nitrogen Fixation in Lotus japonicus Nodules
- Characterization of Uricase Promoter : GUS Gene Expression Using transgenic Lotus japonicus
- Characterization of Uricase Proteins Expressed Using pET32 and Soybean Nodule cDNA Clones
- Characterization of Transgenic Lotus japonicus Containing Sense and Anti-sense Uricase Genes
- Symbiotic Nitrogen Fixation at the Late Stage of Nodule Formation in Lotus japonicus and Other Legume Plants
- Expression of Two Uricase (Nodulin-35) Genes in a Non-Ureide Type Legume, Medicago sativa
- PROMOTER ANALYSIS OF URICASE II (NODULIN 35) GENES TO IDENTIFY THE ELEMENTS FOR NODULE UNINFECTED CELL-SPECIFIC EXPRESSION
- Effect of Exogenous NH^+_4-N Supply on Distribution of Ureide Content in Various Tissues of Alfalfa Plants, Medicago sativa
- 8-25 Short-term effects of ammonium sulfate application on distribution of ureide and amino acid-N in bleeding sap and underground-parts of alfalfa, Medicago sativa : Effect of exogenous N supply on ureide and amino acid-N
- Comparison of uricase II (nodulin 35) gene promoters from soybean, alfalfa and non-legume, Arabidopsis.
- Preparation of a Monoclonal Antibody against Soybean Nodule Uricase (nod-35), and Immunoblot Analysis of the Expression of nod-35 in Tissues of Various Legumes
- PROMOTER ANALYSIS OF PHOSPHOENOL PYRUVATE CARBOXYKINASE GENE IN TRANSGENIC RICE
- ISOLATION AND CHARACTERIZATION OF AN URICASE II (NODULIN 35) GENE FROM ARABIDOPSIS.
- URICASE (NOD-35) GENE EXPRESSION ANALYSIS IN UREIDE AND AMIDE TYPE LEGUME PLANTS
- ANALYSES OF THE PROMOTER REGION OF THE SOYBEAN URICASE GENES USING TRANSGENIC HAIRY ROOT SYSTEM.
- Cloning and Characterization of a Novel Ca^/Calmodulin-Dependent Protein Kinase I Homologue in Xenopus laevis
- Subsite Affinities of β-Glucosidase from Aspergillus sojae on Various Xylooligosaccharides
- Enzymes of purine catabolism in soybean plants
- Expression of a Gene for Uricase II (Nodulin-35) in Cotyledons of Soybean Plants
- The Modes of Action of Two Endo-1, 4-β-_D-Xylanases from Aspergillus sojae on Various Xylooligosaccharides
- Purification and Characterization of Two Xylanases and an Arabinofuranosidase from Aspergillus sojae
- FLUCTUATION OF ENZYME ACTIVITIES RELATED TO NITROGEN FIXATION, C6/Cl RATIO, AND NICOTINAMIDE NUCLEOTIDE CONTENTS DURING SOYBEAN PLANT DEVELOPMENT
- Purification and Characterization of the NADP-Malic Enzyme from Bradyrhizobium japonicum A1017
- PRESENCE AND CHARACTERISTICS OF NADP-MALIC ENZYME IN SOYBEAN NODULE BACTEROIDS
- Protein Profile of Symbiotic Bacteria Mesorhizobium loti MAFF303099 in Mid-growth Phase(Biochemistry & Molecular Biology)
- Copper Metallochaperones are Required for the Assembly of Bacteroid Cytochrome c Oxidase Which is Functioning for Nitrogen Fixation in Soybean Nodules
- Expression of two uricase genes in various tissues of soybean plants
- Expression and characterization of PKL01, an Ndr kinase homolog in Lotus japonicus
- Succinate Metabolism of Isolated Soybean Nodule Bacteroids at Low Oxygen Concentration
- Involvement of a Novel Genistein-Inducible Multidrug Efflux Pump of Bradyrhizobium japonicum Early in the Interaction with Glycine max (L.) Merr
- Effects of Oxygen Concentration and Leghemoglobin on Organic Acid Degradation by Isolated Soybean Nodule Bacteriods