Genetic Diversity of Native Bradyrhizobium Populations in Soybean-Growing Areas of Northern Thailand (Soil Biology)

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概要

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• Native Bradyrhizobium isolates (66 isolates) obtained from traditional soybean-growing areas of northern Thailand were classified using 3 genotypic methods: repetitive extragenic palindromic (REP) sequences, enterobacterial repetitive intergenic consensus (ERIC) sequences together with the polymerase chain reaction (PCR) and mapped restriction site polymorphism (MRSP) analysis of 16S rRNA genes. Sequencing of the 16S rRNA genes was performed for some isolates representing the major REP-ERIC-PCR clusters to acquire data supporting the classifications. The results of the classifications using all these genotypic methods appeared to be consistent, with some minor differences. Five major clusters were obtained by integration of all the classifications: clusters Bj-A (B. japonicum), Bj-B, Brhl, Brh2, and Be (B. elkanii). Two clusters, Brhl and Brh2 were distantly related to B. japonicum USDA110 and B. japonicum strains, but were genetically close to B. liaoningense. Cluster Bj-B was closer to USDA110 than the Brh-clusters but the phylogenetic positions were clearly separated from each other. The cowpea Bradyrhizobium isolates capable of nodulating soybean were distributed and classified into all of these five clusters. The results revealed unique genetic characters of the Thai soybean-bradyrhizobia. The classification of these native bradyrhizobia using REP and ERIC-PCR as well as the serotype pattern of these isolates reflected well the phylogenetic relationships among isolates based on differences in 16S rDNA sequences. Our results also indicated the existence of a relationship between the genotype of the native isolates and sampling locations. Some genotypic-clusters; Brhl, Brh2, and Bj-A were mainly obtained from Chiang Mai Province and a certain cluster, Bj-B from Mac Hong Son Province. This relationship was not observed for cluster Be. In contrast to the sampling locations, no relationship was detected between host cultivars and genotype recovery.

• 社団法人日本土壌肥料学会の論文

著者

• Tajima Shigeyuki

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• MINAMISAWA KIWAMU

  Graduate School of Life Sciences, Tohoku University

• MITSUI Hisayuki

  Graduate School of Life Sciences, Tohoku University

• Bhromsin Ampan

  チェンマイ大農

• Bhromsiri Ampan

  Department Of Soil Science Faculty Of Agriculture Chiang Mai University

• Tajima Shigeyuki

  Faculty of Agriculture, Kagawa University

• Shutsrirung Arawan

  Department Of Soil Science Faculty Of Agriculture Chiang Mai University

• Shutsrirung Arawan

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University

• TAJIMA Shigeyuki

  Kagawa Univ.

• Senoo Keishi

  Department Of Applied Biological Chemistry Division Of Agriculture And Life Sciences The University

• Tajima Shigeyuki

  Kagawa Univ. Kagawa Jpn

• Tajima Shigeysuki

  Department Of Life Science Faculty Of Agriculture Kagawa University

• Yokoyama Tadashi

  Laboratory of Plant Nutrition, Tokyo University of Agriculture and Technology

• Senoo Keishi

  Laboratory of Soil Science, Department of Applied Biological Chemistry, Graduate School of Agricultu

• Sameshima Reiko

  Graduate School of Life Science, Tohoku University

• Hisamatsu Makoto

  Laboratory of Food Chemistry, Faculty of Bioresources, Mie University

• Tajima Shigeyuki

  Faculty Of Agriculture Kagawa University

• Hisamatsu M

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University

• Tajima S

  Faculty Of Agriculture Kagawa University

• Tajima S

  Department Of Life Science Kagawa University

• Tajima Shigeyuki

  Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University

• Tajima Shigeyuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Nagoya University

• Hisamatsu M

  Graduate School Of Life Sciences Tohoku University

• Tajima Shigeyuki

  Fac. Agr. Kagawa University

• Tajima Shigeyuki

  Dept. Of Bioresource Science Faculty Of Agriculture Kagawa University

• Tajima Shigeyuki

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Sameshima Reiko

  Graduate School Of Life Science Tohoku University

• Yokoyama T

  Tokyo University Of Agriculture And Technology

• Minamisawa K

  Graduate School Of Life Sciences Tohoku University

• Yokoyama Tadashi

  Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• Bhromsiri A

  Department Of Soil Science Faculty Of Agriculture Chiang Mai University

• Minamisawa Kiwamu

  Graduate School Of Life Sciences Tohoku Univ.

• Tajima Shigeyuki

  Dept. Bioresource Sience Kagawa Univ.

• Senoo Keishi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Shutsrirung A

  Department Of Soil Science Faculty Of Agriculture Chiang Mai University

• Minamisawa Kiwamu

  Graduate School of Life Science, Tohoku University

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