Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea

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概要

• 論文の詳細を見る

• Three endophytic bacterial strains (Burkholderia sp. no. 25, Enterobacter sp. no. 35 and Klebsiella sp. no. 38) isolated from sweet potato and sugarcane were examined for their ability to incorporate the gene encoding green fluorescent protein (GFP) via conjugation and electroporation. Enterobacter sp. no. 35 and Klebsiella sp. no. 38 were successfully tagged with the gfp gene by conjugation using pTn5kmgfpmut1. Fluorescence microscopic observation of Brassica oleracea inoculated with gfp-tagged endophytes revealed that Enterobacter sp. no. 35 and Klebsiella sp. no. 38 colonized the junctions between the lateral roots and the main roots.

• 日本微生物生態学会の論文

著者

• Saeki Yuichi

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki

• Saeki Yuichi

  Department Of Agriculture And Forest Science Faculty Of Agriculture Miyazaki University

• Yamakawa Takeo

  Department Of Plant Resources Faculty Of Agriculture Kyushu University

• Yamakawa Takeo

  Department Of Agricultural Chemistry Kyushu University

• Yamakawa Takeo

  Kyushu Univ. Fukuoka Jpn

• MINAMISAWA KIWAMU

  Graduate School of Life Sciences, Tohoku University

• Zakria Muhammad

  Faculty Of Agriculture University Of Miyazaki

• ZAKRIA Muhammad

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• AKAO Shoichiro

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• NJOLOMA JOYCE

  Faculty of Agriculture, University of Miyazaki

• TANAKA KAZUNORI

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University

• SHIMIZU TASUKU

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University

• NJOLOMA JOYCE

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University

• SAKAI MASAO

  Department of Plant Resources, Faculty of Agriculture, Kyushu University

• Saeki Yuichi

  Dep. Of Biochemistry And Applied Biosciences Fac. Of Agriculture Miyazaki Univ.

• Saeki Y

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki

• Njoloma Joyce

  Faculty Of Agriculture University Of Miyazaki

• Shimizu Tasuku

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University

• Sakai Masao

  Fac. Of Agriculture Kagoshima Univ.

• Akao S

  National Institute Of Agrobiological Resources

• Akao S

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki

• Sato A

  Faculty Of Agriculture Miyazaki University

• Akao Shoichiro

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University

• Tanaka Kazunori

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University

• Saeki Yuichi

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University

• Akao Shoichiro

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki

• Sakai Masao

  Department Of Chemistry And Bioscience Faculty Of Science Kagoshima University

• Minamisawa Kiwamu

  Graduate School Of Life Sciences Tohoku Univ.

• Sakai Masao

  Department Of Anatomy School Of Medicine Fujita Health University

• Yamakawa Takeo

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University

• Minamisawa Kiwamu

  Graduate School of Life Science, Tohoku University

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