Infection, Multiplication and Evaluation of the Nitrogen-Fixing Ability of Herbaspirillum sp. Strain B501gfp1 in Sugarcane Stems Inoculated by the Vacuum Infiltration Method
スポンサーリンク
概要
- 論文の詳細を見る
The present study was conducted with the aim of finding a suitable delivery method for introducing endophytic bacteria into new sugarcane stalks. Stem pieces of cultivars NiF8 and Ni15 were inoculated with Herbaspirillum sp. strain B501gfp1 by the vacuum infiltration method. The inoculated bacteria showed higher levels of nitrogenase activity in cultivar Ni15 than NiF8. The population density immediately after inoculation (10^7 CPU g [fresh weight]^<-1>) showed that strain B501gfp1 successfully entered the stem tissues of Ni15, where its presence was confirmed by fluorescence microscopic observations. Colonization was observed in the intercellular spaces and between the cell layers. The population of inoculated bacteria remained stable at 5 days after inoculation. The inoculated stems also showed adequately strong acetylene reduction activity compared to uninoculated stems in the absence of indigenous nitrogen-fixing bacteria.
- 日本微生物生態学会の論文
著者
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Saeki Yuichi
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Saeki Yuichi
Department Of Agriculture And Forest Science Faculty Of Agriculture Miyazaki University
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Zakria Muhammad
Faculty Of Agriculture University Of Miyazaki
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Saeki Yuichi
Faculty of Agriculture, University of Miyazaki
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Yamamoto Akihiro
Faculty of Agriculture, University of Miyazaki
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Akao Shoichiro
Faculty of Agriculture, University of Miyazaki
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YAMAMOTO Akihiro
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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UDONISHI KANA
Faculty of Agriculture, University of Miyazaki
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Saeki Yuichi
Dep. Of Biochemistry And Applied Biosciences Fac. Of Agriculture Miyazaki Univ.
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Saeki Y
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Udonishi Kana
Faculty Of Agriculture University Of Miyazaki
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Akao S
National Institute Of Agrobiological Resources
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Akao S
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Sato A
Faculty Of Agriculture Miyazaki University
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Saeki Yuichi
Faculty Of Agriculture University Of Miyazaki
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Akao Shoichiro
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Yamamoto Akihiro
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Akao Shoichiro
Faculty Of Agriculture Miyazaki University
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Saeki Yuichi
Faculty Of Agriculture Miyazaki University
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Yamamoto Akihiro
Dep. Of Biochemistry And Applied Biosciences Fac. Of Agriculture Univ. Of Miyazaki
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