Nitrogen Fixation and Translocation in Young Sugarcane (Saccharum officinarum L.) Plants Associated with Endophytic Nitrogen-Fixing Bacteria
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概要
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The tracer 15N2 was used to investigate sites of N2 fixation and the possible translocation of the fixed N. Young sugarcane plants (Saccharum officinarum L.) from a stem cutting were exposed to 15N2-labeled air in a 500 mL plastic cylinder. Plants fed 15N2 for 7 days were grown in normal air for a further chase period. After 21 days, about half of the N originating in the stem cutting had been transported to the shoot and roots, suggesting that the cutting played a role in supplying N for growth. After 3 days of feeding, the percentage of N derived from 15N2 was higher in the roots (2.22 %) and stem cutting (0.271 %) than the shoot (0.027%). Most of the fixed N was distributed in the 80 % ethanol-insoluble fractions in each plant part, and the 15N fixed either in the roots or in the stem cutting remained there and was not appreciably transported to the shoot. The results were quite different from the fate of fixed N in soybean nodules, which is rapidly transported from nodules to roots and shoots.
- 2009-09-01
著者
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Sueyoshi Kuni
Graduate School Of Science And Technology ; Niigata University
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OHTAKE Norikuni
Graduate School of Science and Technology ; Niigata University
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OHYAMA Takuji
Graduate School of Science and Technology ; Niigata University
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Akao Shoichiro
Faculty of Agriculture, University of Miyazaki
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Sato Takashi
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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MOMOSE ATSUSHI
Graduate School of Science and Technology, Niigata University
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NAKANISHI YASUHIRO
Miyako Subtropical Experimental Farm, Tokyo University of Agriculture
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Momose Atsushi
Graduate School Of Science And Technology Niigata University
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Akao S
National Institute Of Agrobiological Resources
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Akao S
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Sato A
Faculty Of Agriculture Miyazaki University
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Sato T
Akita Prefectural Univ. Akita
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Akao Shoichiro
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Ohtake Norikuni
Dept. Appl. Biol. Chem. Fac. Agric. Niigata Univ.
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Nakanishi Yasuhiro
Miyako Subtropical Experimental Farm Tokyo University Of Agriculture
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Takeda S
Osaka Women's Univ. Osaka Jpn
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Sugimoto T
Plant Nutrition Laboratory Faculty Of Agriculture Kobe University
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Sueyoshi Kuni
Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University
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Sueyoshi Kuni
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Ohyama T
Niigata Univ. Niigata Jpn
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Sato T
Faculty Of Bioresource Sciences Akita Prefectural University
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Sugimoto T
Hayashibara Biochemical Laboratories Inc.
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Akao Shoichiro
Faculty Of Agriculture Miyazaki University
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Sato T
Department Of Biological And Environmental Sciences Akita Prefectural University
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Sato Takashi
Faculty Of Bioresource Sciences Akita Prefectural University
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