Role of Xylem Sap Nitrate in the Regulation of Nitrate Reductase Gene Expression in Leaves of Barley (Hordeurn vulgare L.) Seedlings
スポンサーリンク
概要
- 論文の詳細を見る
The role of xylem sap nitrate in the regulation of nitrate reductase (NR) in leaves was investigated in barley (Hordeum vulgare L.) seedlings. Upon the exposure of barley roots to nitrate at concentrations ranging from 0.1 to 5 mM, rapid accumulation of NADH-specific NR mRNA was observed in both leaves and roots. In the same treatment, the concentration of xylem sap nitrate also rapidly increased during the early feeding period and reached a steady state at concentration ranging from 15 to 50 mM. Patterns of NR mRNA accumulation in leaves were closely linked with the increase in the nitrate concentration in the xylem sap, but not with the amount of nitrate stored in the leaf tissues. High levels of NR mRNA in intact leaves were observed only when the xylem sap nitrate reached mM levels. Supply of different concentrations of nitrate to detached leaves also indicated that mM levels of nitrate supplied through the xylem were required for adequate accumulation of NR mRNA in leaves. When nitrate-grown seedlings were transferred to a nitrate-free medium, a parallel decline in both the concentration of xylem sap nitrate and NR mRNA level was observed, while the nitrate concentrations in leaf tissues remained constant. These results indicate that continuous supply of mM levels of nitrate through the xylem is important for maintaining the high levels of NR mRNA in barley leaves.
- 社団法人日本土壌肥料学会の論文
著者
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Sueyoshi Kuni
Graduate School Of Science And Technology ; Niigata University
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OHTAKE Norikuni
Graduate School of Science and Technology ; Niigata University
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OHYAMA Takuji
Graduate School of Science and Technology ; Niigata University
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Ohyama Takuji
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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OHTAKE Norikuni
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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SUEYOSHI Kuni
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Sato T
Akita Prefectural Univ. Akita
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OJI Yoshikiyo
Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University
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Ohtake Norikuni
Dept. Appl. Biol. Chem. Fac. Agric. Niigata Univ.
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SUGIMOTO Toshio
Department of Biological and Environmental Sciences, Faculty of Agriculture, Kobe University
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ティワリ カウサル
新潟大農
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Kaushal Tewari
Faculty Of Agriculture Niigata University
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Kawachi T
National Institute Of Livestock And Grassland Science
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Sugimoto T
Plant Nutrition Laboratory Faculty Of Agriculture Kobe University
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Sugimoto T
Univ. Tsukuba Tsukuba Jpn
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Sueyoshi Kuni
Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University
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Sueyoshi Kuni
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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Kawachi Tahei
Department of Applied Biological Chemistry, Niigata University
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Shoji Yoshiro
Department of Environmental and Biological Science, Kobe University
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Kleinhofs Andris
Department of Crop and Soil Sciences, Washington State University
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Warner Robert
Department of Crop and Soil Sciences, Washington State University
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Oji Yoshikiyo
Plant Nutrition Laboratory Faculty Of Agriculture Kobe University
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Oji Yoshikiyo
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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Kleinhofs Andris
Department Of Crop And Soil Sciences Washington State University
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Sueyoshi Kuni
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Kaushal T
新潟大 農
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Warner Robert
Department Of Crop And Soil Sciences Washington State University
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Sato Yuki
Fac. Of Agriculture Niigata Univ.
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Sugimoto Toshio
Department Of Biological And Environmental Science Faculty Of Agriculture Graduate School Of Agricul
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Sugimoto T
Hayashibara Biochemical Laboratories Inc.
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OHTAKE Norikuni
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
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