Two-component high-affinity nitrate transport system in barley : Membrane localization, protein expression in roots and a direct protein-protein interaction
スポンサーリンク
概要
- 論文の詳細を見る
A high affinity transport system (HATS) for nitrate in plants is operated by a two-component NRT2/NAR2 transport system. However, the regulation and localization of NRT2 and NAR2 at protein level are largely unknown and especially so in crop plant species. In this study with barley (Hordeum vulgare), membrane localization, protein expression in the roots, and a direct protein-protein interaction of HvNRT2 and HvNAR2 proteins were investigated. Immunochemical analysis showed that both HvNRT2 and HvNAR2 proteins were co-localized in the plasma membrane of the roots. Expression of HvNRT2 and HvNAR2 proteins was more strongly induced by treatment with higher concentrations of external nitrate, while HATS activity and transcripts for HvNRT2 and HvNAR2 were markedly repressed. An affinity column binding analysis using recombinant proteins suggests that the C-terminus of HvNRT2.1 is possibly involved in its binding to the HvNAR2.3 central region and that the Ser463 present in the HvNRT2.1 C-terminus plays a role in the binding ability.
- 2009-03-25
著者
-
Sueyoshi Kuni
Graduate School Of Science And Technology ; Niigata University
-
ISHIKAWA Shinji
Graduate School of Science and Technology ; Niigata University
-
ITO Yuka
Faculty of Agriculture, Niigata University
-
SATO Yuki
Faculty of Agriculture, Niigata University
-
FUKAYA Yuka
Faculty of Agriculture, Niigata University
-
TAKAHASHI Misa
Graduate School of Science, Hiroshima University
-
MORIKAWA Hiromichi
Graduate School of Science, Hiroshima University
-
OHTAKE Norikuni
Graduate School of Science and Technology ; Niigata University
-
OHYAMA Takuji
Graduate School of Science and Technology ; Niigata University
-
Fukaya Yuka
Faculty Of Agriculture Niigata University
-
TAKAHASHI Misa
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
-
Takahashi M
Graduate School Of Sci. Hiroshima Univ.
-
Takahashi Misa
Graduate School Of Science Hiroshima University
-
Takahashi Misa
Graduate Department Of Gene Science Faculty Of Science Hiroshima University
-
森川 弘道
広島大学院・理学研究科
-
Ohtake Norikuni
Dept. Appl. Biol. Chem. Fac. Agric. Niigata Univ.
-
Morikawa H
Graduate School Of Science Hiroshima University
-
Morikawa Hiromichi
Hiroshima Univ. Higashi‐hiroshima Jpn
-
Sueyoshi Kuni
Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University
-
Ito Yuka
Faculty Of Agriculture Niigata University
-
Sueyoshi Kuni
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
-
Ohyama T
Niigata Univ. Niigata Jpn
-
Ishikawa Shinji
Graduate School Of Agriculture And Life Sciences University Of Tokyo
-
Morikawa Hiromichi
Grad. Dept. Gene Sci Fac. Sci. Hiroshima Univ.
-
Morikawa Hiromichi
Graduate Department Of Gene Science Faculty Of Science Hiroshima University
-
Sato Yuki
Faculty Of Agriculture Niigata University
関連論文
- Two-component high-affinity nitrate transport system in barley : Membrane localization, protein expression in roots and a direct protein-protein interaction
- Nitrogen Fixation and Translocation in Young Sugarcane (Saccharum officinarum L.) Plants Associated with Endophytic Nitrogen-Fixing Bacteria
- Development of Des-Fatty Acyl-Polymyxin B Decapeptide Analogs with Pseudomonas aeruginosa-Specific Antimicrobial Activity
- Contribution of Each Amino Acid Residue in Polymyxin B_3 to Antimicrobial and Lipopolysaccharide Binding Activity
- Des-Fatty Acyl-Polymyxin B Decapeptide Analogs with Antimicrobial Activity Specifically against Pseudomonas aeruginosa
- Semi-synthesis of Polymyxin B (2-10) and Colistin (2-10) Analogs Employing the Trichloroethoxycarbonyl (Troc) Group for Side Chain Protection of α, γ-Diaminobutyric Acid Residues
- Chemical Conversion of Natural Polymyxin B and Colistin to Their N-Terminal Derivatives
- Semi-Synthesis of Polymyxin B-(2-10) Analogs Employing the Trichloroethoxycarbonyl (Troc) Group for Side Chain Protection of 2,4-Diaminobutyric Acid Residues
- Structure-Activity Relationships of Neuromedin U. V. Study on the Stability of Porcine Neuromedin U-8 at the C-Terminal Asparagine Amide under Mild Alkaline and Acidic Conditions
- Antimicrobial Activity of Des-Fatty Acyl-Polymyxin B Decapeptide N-Terminal Analogs
- The Contribution of the N-Terminal Structure of Polymyxin B Peptides to Antimicrobial and Lipopolysaccharide Binding Activity
- Antimicrobial Activity of Polymyxin B_3 Analogs modified at the Hydrophobic Amino Acids
- Transient Expression of β-Glucuronidase in Plastids of Various Plant Cells and Tissues Delivered by a Pneumatic Particle Gun
- Bombardment-Mediated Transformation of Plant Cells
- Effects of ion beam irradiation on the regeneration and morphology of Ficus thunbergii Maxim
- Isolation and characterization of a novel Arabidopsis thaliana mutant that shows low nitrate concentration in shoots(Plant Nutrition)
- Absorption and translocation of nitrogen in cucumber (Cucumis sativus L.) plants using the ^N tracer technique(Plant Nutrition)
- Analysis of the nitrogen nutrition of soybean plants with deep placement of coated urea and lime nitrogen(Plant Nutrition)
- Characteristics of initial growth of hypernodulation soybean mutants, NOD1-3, NOD2-4 and NOD3-7, affected by inoculation of bradyrhizobia and nitrate supply(Plant Nutrition)
- Effect of nitrogen supply on nitrogen and carbohydrate constituent accumulation in rhizomes and storage roots of Curcuma alismatifolia Gagnep.(Plant Nutrition)
- Comparison of the depth of placement of lime nitrogen on growth, N_2 fixation activity, seed yield and quality of soybean (Glycine max (L.) Merr.) plants(Plant Nutrition)
- Allocation of photosynthetic products in soybean during the early stages of nodule formation(Plant Nutrition)
- Determination of the uptake and utilization of nitrogen in Curcuma alismatifolia Gagnep. using ^N isotope(Plant Nutrition)
- Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino
- Long-Term Effect of Nitrate Application from Lower Part of Roots on Nodulation and N_2 Fixation in Upper Part of Roots of Soybean (Glycine max (L.) Merr.) in Two-Layered Pot Experiment(Plant Nutrition)
- ^N Analysis of the Promotive Effect of Deep Placement of Slow-Release N Fertilizers on Growth and Seed Yield of Soybean (Plant Nutrition)
- Effect of Placement of Urea and Coated Urea Fertilizers on Yield and Quality of Soybean (Glycine max (L.) Merr.) Seeds(Plant Nutrition)
- Contribution of Shoots and Roots to In Vivo Nitrate Reduction in NADH-Specific Nitrate Reductase-Deficient Mutant Seedlings of Barley (Hordeum vulgare L.)(Plant Nutrition)
- Quantitative and Isotopic Analysis of Amino Acids, Allantoin, and Allantoic Acid in Soybeans by LC-MS Using the Atmospheric Pressure Chemical lonization Method(Plant Nutrition)
- Systemic and Local Effects of Long-Term Application of Nitrate on Nodule Growth and N_2 Fixation in Soybean (Glycine max [L.] Merr.) (Plant Nutrition)
- Site of Nitrogen Accumulation in Tulip (Tulipa gesneriana L.) Roots during Winter
- Site of Nitrogen Accumulation in Tulip (Tulipa gesneriana L.) Roots during Winter (Plant Nutrition)
- Photoassimilate Partitioning in Hypernodulation Mutant of Soybean (Glycine max (L.) Merr.) NOD1-3 and Its Parent Williams in Relation to Nitrate Inhibition of Nodule Growth (Plant Nutrition)
- Effects of Anion Channel Blockers on Xylem Nitrate Transport in Barley Seedlings
- Effect of Exogenous NH^+_4-N Supply on Distribution of Ureide Content in Various Tissues of Alfalfa Plants, Medicago sativa
- 8-25 Short-term effects of ammonium sulfate application on distribution of ureide and amino acid-N in bleeding sap and underground-parts of alfalfa, Medicago sativa : Effect of exogenous N supply on ureide and amino acid-N
- Monoclonal Antibodies Spicific for NADH-Ferricyanide Reductase Domain of Spinach Leaf Nitrate Reductase
- CHARACTERIZATION OF SAR SEQUENCE FLANKING ARABIDOPSIS NITRITE REDUCTASE GENE
- CHARACTERIZATION OF TRANSGENICARABIDOPSIS THALIANA PLANTS ENRICHED WITH THE NIR GENE
- Rapid and Reversible Nitrate Inhibition of Nodule Growth and N_2 Fixation Activity in Soybean (Glycine max (L.) Merr.)
- Possible Involvement of Photosynthetic Supply in Changes of Nodule Characteristics of Hypernodulating Soybeans
- INACTIVATION OF BARLEY NITRATE REDUCTASE BY 14-3-3 PROTEIN
- Determination of Leghemoglobin Components and Xylem Sap Composition by Capillary Electrophoresis in Hypernodulation Soybean Mutants Cultivated in the Field
- Regulation of Accumulation of β-Subunit of β-Conglycinin in Soybean Seeds by Nitrogen
- Real-time imaging of nitrogen fixation in an intact soybean plant with nodules using ^N-labeled nitrogen gas(Plant Nutrition)
- A High Molecular Weight Glutamyl Endopeptidase and Its Endogenous Inhibitors from Cucumber Leaves
- ANALYSIS OF GENES INVOLVED IN THE METABOLISM OF (NO)_2 IN ARABIDOPSIS THALIANA
- Effect of Exogenous Salicylic Acid Supply on Nodule Formation of Hypernodulating Mutant and Wild Type of Soybean
- Regeneration and Transformation of a Roadside Tree Pittosporum tobira A.
- Novel Des-Fatty Acyl-Polymyxin B Derivatives with Pseudomonas aeruginosa-Specific Antimicrobial Activity
- Temporary Application of Nitrate to Nitrogen-Deficient Soybean Plants at the Mid-to Late-Stages of Seed Development Increased the Accumulation of the β-Subunit of β-Conglycinin, a Major Seed Storage Protein
- Measurement Method of Gytokinis in Soybean Immature Seed using Fluorescent Derivative and LC/MS System.
- 6 Comparative studies on contribution of roots and shoots to in vivo nitrate reduction in wild type and nitrate reductase-deficient mutant of barely
- Role of Xylem Sap Nitrate in the Regulation of Nitrate Reductase Gene Expression in Leaves of Barley (Hordeurn vulgare L.) Seedlings
- Effect of Short-Term Application of Nitrogen on the Accumulation of β-Subunit of β-Conglycinin in Nitrogen-Starved Soybean(Glycine max L.) Developing Seeds
- REGULATION OF NITRATE REDUCTASE GENE EXPRESSION IN BARLEY : 2. ROLE OF ANION CHANNEL
- REGULATION OF NITRATE REDUCTASE GENE EXPRESSION IN BARLEY : 1. ROLE OF XYLEM SAP NITRATE
- EXPRESSION OF ASPARAGINE SYNTHETASE IN GERMINATING RICE SEED
- Effect of Deep Placement of Calcium Cyanamide, Coated Urea, and Urea on Soybean (Glycine max (L.) Merr.) Seed Yield in Relation to Different Inoculation Methods
- Effects of Inhibitors for Signaling Components on the Expression of the Genes for Nitrate Reductase and Nitrite Reductase in Excised Barley Leaves
- EFFECTS OF INHIBITORS ON EXPRESSION OF NITRATE REDUCTASE GENE IN BARLEY
- FUNCTION OF PROTEASES EXPRESSING INCUCUMBER LEAF
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN DERIVED FROM NITROGEN DIOXIDE ABSORBED IN PLANT LEAVES
- TIMING OF THE LOCALIZATION OF p34^ IN PREPROPHASE BANDS OF ONION ROOT TIPS
- Determination of Levels of NO_3^-,NO_2^- and NH_4^+ Ions in Leaves of Various Plants by Capillary Electrophoresis
- Cloning and Nucleotide Sequence of a Leaf Ferredoxin-Nitrite Reductase cDNA of Rice
- The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic arabidopsis plants
- Ureide and Amino Acids in Xylem Sap of Soybean (Glycine max L.) Are Affected by Both Nodulation and Nitrogen Supply from Soil
- Ureide and Amino Acids in Xylem Sap of Soybean (Glycine max L.) Are Affected by Both Nodulation and Nitrogen Supply from Soil (Plant Nutrition)
- Phytoremediators from abandoned rice field
- IJ1 is an orientation-independent transformation enhancer sequence
- Development of Regeneration and Transformation Systems for Raphiolepis umbellata L. Plants Using Particle Bombardment
- Mechanism of Transgene Integration into a Host Genome by Particle Bombardment
- Identification of Long Needle Crystals and Distribution of Oxalate Salts in Narcissus cv. 'Garden Giant'
- Nitrate Transport Pathway into Soybean Nodules Traced by Tungstate and ^NO^-_3
- Antimicrobial Activity of Various Aminocyclohexylcarbonyl-polymyxin B (2-10) Derivatives
- Comparison of phosphoenolpyruvate carboxylases in seeds of maize (Zea mays) and rice (Oryza sativa)
- d14, a Strigolactone-Insensitive Mutant of Rice, Shows an Accelerated Outgrowth of Tillers
- Suppression of Tiller Bud Activity in Tillering Dwarf Mutants of Rice
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN (UN) DERIVED FROM NITROGEN DIOXIDE ABSORBED IN PLANT LEAVES
- METABOLISM OF NITROGEN OXIDES IN PLANTS-FORMATION OF UN COMPOUNDS AND N_2O
- ASSIMILATION OF NITROGEN DIOXIDE IN TRANSGENIC PLANTS (3) NR AND GS EXPRESSORS
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN DERIVED FROM NO_2 ABSORBED IN PLANT LEAVES (3) PURIFICATION AND ANALYSIS OF MAIN COMPOUNDS
- ANALYSIS OF COMPOUNDS BEARING UNIDENTIFIED NITROGEN DERIVED FROM NO_2 ABSORBED IN PLANT LEAVES
- PROTEINS EXPRESSED IN RESPONSE TO FUMIGATION WITH NITROGEN DIOXIDE IN PLANT LEA VES
- EMISSION OF N_2O FROM HIGHER PLANTS (2) ROLE OF PHOTOSYNTHETIC ELECTRON FLOW
- Expression of the β-Glucuronidase Gene Introduced into Intact Leaves Attached to Arabidopsis thaliana Plants by Particle Gun
- Emergence of Proteases in Germinating Cucumber Cotyledons and Their Roles in the Two-Step Degradation of Storage Protein
- Purification of Two Major Proteases in Cucumber Leaves
- Properties and substrate specificities of proteolytic enzymes from the edible basidiomycete Grifola frondosa(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- The Autoregulation of Nodulation Mechanism is Related to Leaf Development
- Effect of basal side-dressing of various types of coated urea fertilizer on shoot growth, yield components and seed composition of soybean (Glycine max (L.) Merr.)(Soil Chemistry and Mineralogy)
- Amino Acid Composition in Xylem Sap of Soybean Related to the Evaluation of N_2 Fixation by the Relative Ureide Method
- Lack of β-Subunit of β-Conglycinin in Non-Nodulating Isolines of Soybean
- High Frequency Stable Transformation of Arabidopsis thaliana by Particle Bombardment
- GMO research in Japan-the state of the art
- REGULATION OF β-CONGLYCININ ACCUMULATION IN SOYBEAN SEEDS BY NITROGEN DEFICIENCY AND HORMONE LEVELS
- Heterogeneous expression and emulsifying activity of class I hydrophobin from Pholiota nameko
- Prolonged exposure to atmospheric nitrogen dioxide increases fruit yield of tomato plants
- Quantitative analysis of the initial transport of fixed nitrogen in nodulated soybean plants using ^N as a tracer(Plant nutrition)