Characterization of Plasma Membrane-Bound Fe^<3+>-Chelate Reductase from Fe-Deficient and Fe-Sufficient Cucumber Roots
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概要
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In vivo Fe^<3+>-chelate reductase (FeR) activity in cucumber roots (Cucumis sativus L.) increased by transferring the plants from Fe-sufficient (+Fe) conditions to Fe-deficient (-Fe) conditions. This increase was inhibited by the protein synthesis inhibitor, cycloheximide. The plasma membranes were isolated from the +Fe and -Fe roots and then the enzymatic properties of plasma membrane-bound FeR were characterized. The FeR in the plasma membranes from both the +Fe and -Fe roots reduced Fe^<3+>-citrate using NADH as an electron donor in the presence of Triton X-100. Plasma membrane-bound FeR from both types of roots showed similar Km values for Fe^<3+>-citrate and NADH at 70 and 100 μM, respectively, whereas V_max of the enzyme from -Fe roots was three-fold higher than that of the enzyme from +Fe roots. The enzyme was solubilized from plasma membranes with 1.0% Triton X-100 and subsequently analyzed on an isoelectric focusing gel. The activity staining of the gel after electrophoresis showed that four FeR isozymes with different pIs of 5.3, 6.8, 7.5, and 8.7 were present on the plasma membranes of both the +Fe and -Fe roots. Only the intensity of the pI 7.5 band was enhanced in the -Fe roots. These results suggested that the increase of FeR activity in the -Fe roots resulted from the increased synthesis of the FeR isoform which is constitutively present in the plasma membranes of +Fe roots.
- 社団法人日本土壌肥料学会の論文
著者
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SUEYOSHI Kuni
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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OJI Yoshikiyo
Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University
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Sueyoshi Kuni
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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Oji Yoshikiyo
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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Hirata Osamu
Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University
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Hirata Osamu
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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