Changes in DNA Level of Growing Barley Seedlings
スポンサーリンク
概要
- 論文の詳細を見る
The DNA content of barley (Hordeum vulgare L. cv. Daisen-gold) seedlings was determined by fluorometry with a fluorescent dye, 4', 6-diamidino-2-phenyl-indole (DAPI) based on the method developed by Baer et al. (Plant Physiol., 70, 999-1003, 1982). Extraction and determination of DNA were investigated for application to barley seedlings. NaCl (2.0 M) and Sarkosyl (1%) were required in the homogenization buffer to obtain a higher DNA yield. The rate of increase of the content of DNA in growing barley seedlings was lower than that of fresh and dry weight. the distribution of DNA in a barley plant was analyzed. Higher DNA contents were found in the second leaf and root tip region. The relationship between the DNA content and cell volume was discussed.
- 社団法人日本土壌肥料学会の論文
著者
-
WAKIUCHI Nariaki
Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University
-
OJI Yoshikiyo
Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University
-
SHIGA Hitoichi
Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University
-
Oji Yoshikiyo
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
-
Shiga H
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
-
Mukai Norio
Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University
-
Kubo Shigeo
Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University
-
Kubo Shigeo
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
-
Mukai Norio
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
-
Wakiuchi Nariaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
関連論文
- CHARACTERIZATION OF PROTON PUMPING AND ATPase ACTIVITIES IN MICROSOMAL FRACTIONS FROM BARLEY ROOTS
- A High Molecular Weight Glutamyl Endopeptidase and Its Endogenous Inhibitors from Cucumber Leaves
- Role of Xylem Sap Nitrate in the Regulation of Nitrate Reductase Gene Expression in Leaves of Barley (Hordeurn vulgare L.) Seedlings
- Reconstitution and Characterization of Plasma Membrane H^+-ATPase Activity from Rice Roots
- Effects of Inhibitors for Signaling Components on the Expression of the Genes for Nitrate Reductase and Nitrite Reductase in Excised Barley Leaves
- Properties of the Vanadate-Sensitive ATPase of the Plasma Membrane Vesicles from Rice Roots
- Characterization of Plasma Membrane-Bound Fe^-Chelate Reductase from Fe-Deficient and Fe-Sufficient Cucumber Roots
- Extraction and Affinity Purification of NADH : Nitrate Reductase from Barley (Hordeum distichum L.) Roots
- Changes in DNA Level of Growing Barley Seedlings
- NITRITE REDUCTION SYSTEM IN BARLEY ROOTS : EXISTENCE OF AN ELECTRON CARRIER OPERATING IN AN ELECTRON-TRANSPORT SEQUENCE FROM GLUCOSE-6-PHOSPHATE TO NITRITE REDUCTASE
- New evidence for the metabolic conversion of aspartate-4-^C to glutamine-1-^C during the initial period of ammonia assimilation in the roots of barley plants
- Inverse Correlation of Thiol Proteinase with Nitrate Reductase Activities in Barley Leaves
- Action of Thiol Proteinase on Nitrate Reductase in Leaves of Hordeum distichum L.
- Purification and Characterization of Thiol Proteinase as a Nitrate Reductase-Inactivating Factor from Leaves of Hordeum distichum L.
- A Nitrate Reductase-Inactivating Factor from Barley (Hordeum distichum L.) Leaves
- Quantitative changes of free amino acids and amides in barley plants during ammonia and nitrate assimilation
- Rapid synthesis of glutamine during the initial period of ammonia assimilation in roots of barley plants
- Enzymatic Determination of Propylene Glycol in Commercial Foods
- Application of Nitrate Reductase for the Determination of Nitrate in Meat and Fishery Products