Identification of Possible Intermediates in in Vivo Degradation of Spinach Nitrate Reductase
スポンサーリンク
概要
- 論文の詳細を見る
Degradation intermediates of nitrate reductase (NR) were extracted from fresh spinach leaves in the presence of protease inhibitors, and concentrated by chromatography on an anti-NR IgG-conjugated Sepharose-4B column. Seven polypeptides, 120 (that of the intact subunit), 110, 100, 76, 64, 62, and 44 kDa, were obtained. Their cross-reactivities to mono-specific polyclonal antibody and monoclonal antibody prepared against NR subunit were assessed by an immunoblotting method. All polypeptides were recognized with mono-specific polyclonal antibody. On the other hand, four of them, at 120, 110, 100, and 44 kDa, were recognized with monoclonal antibody which specifically binds to the NADH-ferricyanide reductase domain of NR. The possibility that these polypeptides were artifacts in preparation was ruled out by an internal tracer experiment. Thus, it is concluded that spinach leaf NR in vivo degraded via putative intermediates catabolic products of 110, 100, 76, 64, 62, and 44 kDa.
- 社団法人 日本農芸化学会の論文
著者
-
Ogura Nagao
Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University
-
Nakagawa Hiroki
Department Of Agricultural Chemistry Faculty Of Agriculture Saga University
-
Sueyoshi Kuni
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
-
SUEYOSHI Kuni
Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University
関連論文
- A Nitrate Reductase Inactivator Protein from Spinach. Purification, Molecular Weight and Subunit Composition
- Determination of the uptake and utilization of nitrogen in Curcuma alismatifolia Gagnep. using ^N isotope(Plant Nutrition)
- Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino
- Inhibition of Proteasome by MG-132 Treatment Causes Extra Phragmoplast Formation and Cortical Microtubule Disorganization during M/G_1 Transition in Synchronized Tobacco Cells
- Overexpression of a gene for 26S proteasome subunit RPN10 confers enhanced resistance to canavanine, an analog of arginine, in transgenic rice (Oryza sativa L.)
- Cell-cycle-related variation in proteins in suspension-cultured rice cells
- Cell-Cycle Dependent Dynamic Change of 26S Proteasome Distribution in Tobacco BY-2 Cells
- Cloning and Sequencing of cDNA from Oryza sativa encoding a homolog to non-ATPase subunit,MBP1, of 26S Proteasome in Arabidopsis thaliana
- Changes in Proteasome Levels in Spinach (Spinacia oleracea) Seeds during Imbibition and Germination
- Immuno-gold Localization of Nitrate Reductase in Spinach (Spinacia oleracea) Leaves
- Isolation and Some Properties of a 115-Kilodalton Nitrate Reductase-Inactivator Protein from Spinacia oleracea
- Control by Glutamine of the Synthesis of Nitrate Reductase in Cultured Spinach Cells : PROTEINS, ENZYMES AND METABOLISM
- Size and Levels of mRNA for Acid Invertase in Ripe Tomato Fruit
- Immunological Characterization of Nitrate Reductase in Different Tissues of Spinach Seedlings
- Polygalacturonase mRNA of Tomato : Size and Content in Ripe Fruits
- Cell-Free Synthesis of a Putative Precursor of Polygalacturonase in Tomato Fruits
- Effect of Heat Treatment on the Development of Polygalacturonase Activity in Tomato Fruit during Ripening
- Monoclonal Antibodies Spicific for NADH-Ferricyanide Reductase Domain of Spinach Leaf Nitrate Reductase
- Isoforms of Invertase in Grape Berries(Biological Chemistry)
- Involvement of Proteasome-Ubiquitin System in Wound-Signaling in Tobacco Plants
- Role of Xylem Sap Nitrate in the Regulation of Nitrate Reductase Gene Expression in Leaves of Barley (Hordeurn vulgare L.) Seedlings
- Reconstitution and Characterization of Plasma Membrane H^+-ATPase Activity from Rice Roots
- Effects of Inhibitors for Signaling Components on the Expression of the Genes for Nitrate Reductase and Nitrite Reductase in Excised Barley Leaves
- Quantitative Anhydrous Mercaptolysis of Algal Galactans Followed by HPLC of Component Sugars
- Purification and Characterization of the Alkaline Serine Protease Produced by Bacillus sp.N4 Strain from Fish Skin Mucus
- Effect of flunitrazepam on sleep and memory
- Alcohol effect on sleep electroencephalography by fast Fourier transformation
- Characterization of Plasma Membrane-Bound Fe^-Chelate Reductase from Fe-Deficient and Fe-Sufficient Cucumber Roots
- Sequence Analysis of Cloned cDNA and Proteolytic Fragments for Nitrate Reductase from Spinacia oleracea L.
- Chlorate Reducing Activity of Spinach Nitrate Reductase
- Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
- Purification and Some Properties of Peroxidase from Kiwifruit(Biological Chemistry)
- Changes of Polyuronides in Kiwifruit during Ripening(Food & Nutrition)
- Detection of Polygalacturonase in Kiwifruit during Ripening
- Purification and Characterization of Glycoprotein from the Skin Mucus of the Rainbow Trout, Salmo gairdneri
- Structural Characterization of the Carbohydrate Chains from the Skin Mucus Sialoglycoprotein of the Stingray Dasyatis akajei
- Isolation and Properties of a Sialoglycoprotein from the Skin Mucus of the Stingray Dasyatis akajei
- Studies on chlorophyllase in tea leaves III. Properties of soluble and insoluble chlorophyllases
- Purification and Characterization of α-Mannosidase and β-Ν-Acetylhexosaminidase from Watermelon Fruit (Biological Chemistry)
- Selective Hydrolysis of the 3,6-Anhydrogalacotosidic Linkage in Red Algal Galactan : A Combination of Reductive Acid Hydrolysis and Anhydrous Mercaptolysis
- A Novel Sol-Gel Approach to Highly Condensed Silicas at Low Temperature
- Site of free amino acid accumulation in tulip (Tulipa gesneriana) roots during autumn and winter(Plant nutrition)
- Immunological Properties of Two Acid Phosphatases from Tomato Fruit
- Effect of Heat Treatment on Changes in Acid Phosphatase Activity during Tomato Ripening
- Identification of Possible Intermediates in in Vivo Degradation of Spinach Nitrate Reductase