A Nitrate Reductase Inactivator Protein from Spinach. Purification, Molecular Weight and Subunit Composition

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概要

• 論文の詳細を見る

• A nitrate reductase-inactivator protein has been purified 16,000-fold from spinach leaves by pH 5 treatment, chromatography on SE53, Con A-Sepharose, and chromatofocusing. The yield was 12%, the specific activity was 115 units mg^<-1>. Polyacrylamide gel electrophoresis of the final purified inactivator fraction yielded 2 major protein bands and both bands exhibited nitrate reductase-inactivator activity. Analysis of this inactivator protein by gel filtration and SDS-gel electrophoresis revealed protein stainable material only in a molecular weight range of 110,000-115,000. SDS gel electrophoresis under reducing conditions yielded 2 protein bands corresponding to molecular weights of 5 1,000 and 53,000. The proteolytic mapping for the two separated subunits appeared similar and possibly identical.

• 日本植物生理学会の論文

著者

• Yoshimura Tomoaki

  Department Of Pharmacy Ogaki Municipal Hospital

• Yoshimura Tomoaki

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Yoshimura Tomoaki

  Department Of Agricultural Chemistry Faculty Of Horiculture Chiba University

• Sekino Naoko

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Okuo Katsuhiko

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Sato Takahide

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Ogura Nagao

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Nakagawa Hiroki

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• SATO Takahide

  Graduate School of Science and Technology, Chiba University

• Ogura N

  Division Of Bioproduction Science Faculty Of Horticulture Chiba University

• Ogura N

  Chiba Univ. Chiba Jpn

• Ogura Nagao

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Sekino Naoko

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Sato T

  Fac. Horti. Chiba Univ.

• Sato Takahide

  Graduate School Of Science And Technology Chiba University

• Okuo Katsuhiko

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Nakagawa H

  Graduate School Of Science And Technology Chiba University

• Nakagawa Hiroki

  Fac.hort Chiba Univ.

• Nakagawa Hiroki

  Department Of Agricultural Chemistry Faculty Of Agriculture Saga University

• Nakagawa Hiroki

  Dep. Of Applied Biochemistry And Food Sciences Fac. Of Agriculture Saga Univ.

• Ogura N

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Sato Takenhito

  Department Of Immunology Tokai University School Of Medicine

• Sato Tsutomu

  Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And

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